Fast and innovative methodology to monitors the addition of soybean oil in extra virgin olive oil was developed employing ESI-MS with ionization operating in positive mode. A certified extra virgin olive oil and refined soybean oil samples were analyzed by direct infusion, the identification of a natural lipid marker present only in soybean oil (m/z 886.68 [TAG+NH]) was possible. The certified extra virgin olive oil was purposely adulterated with soybean oil in different levels (1, 5, 10, 20, 50, 70, 90%) being possible to observe that the new methodology is able to detect even small fraud concentration, such as 1% (v/v). Additionally, commercial samples were analyzed and were observed the addition of soybean oil as a common fraud in this segment. This powerful analytical method proposed could be applied as routine analysis by control organization, as well as food industries, considering its pronounced advantages; simplicity, rapidity, elevated detectability and minor amounts of sample and solvent consumed.
Depression is increasingly present in the population, and its pathophysiology and treatment have been investigated with several animal models, including olfactory bulbectomy (Obx). Fish oil (FO) supplementation during the prenatal and postnatal periods decreases depression-like and anxiety-like behaviors. The present study evaluated the effect of FO supplementation on Obx-induced depressive-like behavior and cognitive impairment. Female rats received supplementation with FO during habituation, mating, gestation, and lactation, and their pups were subjected to Obx in adulthood; after the recovery period, the adult offspring were subjected to behavioral tests, and the hippocampal levels of brain-derived neurotrophic factor (BDNF), serotonin (5-HT) and the metabolite 5-hydroxyindoleacetic (5-HIAA) were determined. Obx led to increased anxiety-like and depressive-like behaviors, and impairment in the object location task. All behavioral changes were reversed by FO supplementation. Obx caused reductions in the levels of hippocampal BDNF and 5-HT, whereas FO supplementation restored these levels to normal values. In control rats, FO increased the hippocampal level of 5-HT and reduced that of 5-HIAA, indicating low 5-HT metabolism in this brain region. The present results indicate that FO supplementation during critical periods of brain development attenuated anxiety-like and depressive-like behaviors and cognitive dysfunction induced by Obx. These results may be explained by increased levels of hippocampal BDNF and 5-HT, two major regulators of neuronal survival and long-term plasticity in this brain structure.
RESUMO -Este estudo foi realizado para determinação da composição centesimal e do perfil de ácidos graxos do camarão-d'água-doce Macrobrachium amazonicum visando à análise de seu potencial nutritivo. Seis lotes (100 g cada um) de camarões inteiros e com massas individuais de 0,9 a 1,2 g foram triturados, armazenados e, posteriormente, analisados quanto aos teores de umidade, cinzas, PB e lipídios totais (70,3; 1,5; 24,8 e 1,5%, respectivamente). Foram detectadas 36 variedades de ácidos graxos, sendo o mais freqüente o ácido palmítico-16:0 (18,2%). Entre os da série ômega-3, os mais encontrados foram o eicosapentaenóico-20:5n-3, di-homo-alfa-linolênico-20:3n-3, docosahexaenóico-22:6n-3 e alfa-linolênico-18:3n-3 (frequências de 13,9; 9,5; 6,8 e 4,2%, respectivamente). As freqüências de somatórias de ácidos graxos foram de 36,9% para os ômega-3 e de 46,8% para os poliinsaturados. As razões entre os ácidos graxos ômega-6/ômega-3 (n-6/n-3) e os ácidos graxos poliinsaturados/ácidos graxos saturados (AGPI/AGS) foram de 0,3 e 1,6, respectivamente. Todos os parâmetros observados indicaram bom potencial nutritivo para a carne do camarão-d'água-doce. Palavras-chave: ácidos graxos, camarão, composição centesimal, Macrobrachium amazonicum Centesimal composition and fatty acids profile of freshwater prawnABSTRACT -The aim of this work was to determine the centesimal composition and fatty acids profile of freshwater prawn (Macrobrachium amazonicum) for determination of its nutrient potential. Six lots (each one with 100 g) containing whole prawns with individual mass ranging from 0.9 to 1.2 g were triturated and stored for posterior chemical analysis. The average values for moisture, ash, CP and total lipids were as follows: 70.3, 1.5, 24.8, and 1.5%, respectively.It was detected 36 varieties of fatty acids, and the most abundant was the palmitic -16:0 acid with 18.2%. The major omega-3 fatty acids were: eicosapentaenoic-20:5n-3, dihomo-alpha-linolenic-20:3n-3, docosahexaenoic-22:6n-3 and alpha-linolenic-18:3n-3 with frequencies of 13.9, 9.5, 6.8, and 4.2%, respectively. The total frequencies of fatty acids were of 36.9% for omega-3 and 46.8% for polyunsaturated fatty acids. The omega-6/omega-3 (n-6/n-3) ratio and polyunsatured to satured (PUFA/SFA) ratio were of 0.3 and 1.6, respectively. All parameters indicated a good nutritional value of the M.amazonicum meat.
Total phenolic content (TPC), total flavonoids (TF), total anthocyanins (TA), and antioxidant capacity of different parts of four unexplored fruits from Brazil (Syzygium cumini Lam, Solanum nigrum Linn, Inga edulis Mart, and Hovenia dulcis Thunb) were determined; the bioactive compounds and fatty acids were quantified and identified by high-performance liquid chromatography and gas chromatography, respectively. S. cumini peels contained the most TA (63.31 mg/100 g), whilst H. dulcis pulp and peels had the highest TPC (518.18 mg GAE/100 g) and TF (76.54 mg EQ/g). Phenolic compounds responsible for antioxidant capacity of fruits were gallic acid, ellagic acid, kaempferol, and epicatechin. H. dulcis seed showed the highest level of the essential fatty acid omega-3 (3985.95 mg/100 g). PCA showed that PC1 and PC2 explained 90.43% of the total variability of the antioxidant data. Most of the seeds showed omega-3, omega-6, and omega-9 fatty acids at significant concentrations, with two PCs explaining 93.80% of the total variance of the fatty acid contents.
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