No abstract

SummaryPropolis has a long history of use in traditional medicine dating back to at least 300 BC, and has been reported to have a broad spectrum of biological activities. Since most studies have to date focused on medical uses of propolis, its antimicrobial activity against honey bee diseases has been little studied. One of the aims of this study was therefore to investigate the potential use of propolis in honey bee health, especially against American foulbrood, which causes much damage in the beekeeping industry. The second aim was to reveal the different antimicrobial activities of propolis collected from different geographical areas from the neighbouring countries of Greece and Turkey. Propolis samples collected from several regions of Greece and Turkey were investigated for their in vitro antimicrobial activities against Paenibacillus larvae.Eighteen ethanol extracts of propolis (EEP), (nine from Greece and nine from Turkey) were tested for antimicrobial activities against ten P. larvae isolates. The results showed that all P. larvae strains were susceptible to propolis extracts from both Greece and Turkey Furthermore 50 % concentrations of EEP caused significantly wider inhibition zones (P≤0.05) around the discs. Comparisons of the content, and locations and botanical origins of EEPs from Greece and Turkey showed that the kind of vegetation is more important factor than geographic location for their antimicrobial activity. This is the first comprehensive study concerning the antimicrobial activity of propolis samples collected from a wide area around the Aegean Sea. Comparación de la actividad de propóleos de Turquía y Grecia frente a Paenibacillus larvae ResumenEl propóleos tiene una larga historia de uso en la medicina tradicional que se remonta por lo menos a 300 años antes de Cristo, y se ha descrito que tienen un amplio espectro de actividades biológicas. Como la mayoría de los estudios hasta la fecha se han centrado en los usos médicos de los propóleos, su actividad antimicrobiana frente a enfermedades de las abejas de la miel ha sido poco estudiada. Por lo tanto, uno de los objetivos de este estudio fue investigar el posible uso de los propóleos en la salud de la abeja de la miel, especialmente contra la loque americana, que causa mucho daño en la industria de la apicultura. El segundo objetivo era revelar las diferentes actividades antimicrobianas de propóleos recolectados de diferentes áreas geográficas de los países vecinos de Grecia y Turquía. Se investigó la actividad antimicrobiana in vitro de muestras de propóleos recolectados en varias regiones de Grecia y Turquía contra Paenibacillus larvae. Se probó la actividad antimicrobiana de dieciocho extractos de propóleos en etanol (EEP), (nueve de Grecia y nueve de Turquía) contra diez aislamientos de P. larvae. Los resultados mostraron que todas las cepas de P. larvae fueron susceptibles a los extractos de propóleos tanto de Grecia como de Turquía. Además concentraciones al 50% de EEP causaron zonas de inhibición significativamente más amplias (p ...

We aimed to determine the differences and similarities between Greek and Turkish propolis with respect to their chemical composition given that the two countries have many similarities in floral biodiversity. We observed that: a) Greek propolis is different from the European-type propolis, having a high terpene content; therefore we can definitely characterize it as a Mediterranean type; b) the Turkish propolis collected along the coast line of the Aegean Sea is similiar to the examined Greek propolis; c) the remaining Turkish samples, originating from the European part of Turkey, were found to be similiar to the European-type propolis, having a high flavonoid content. Finally, especially two compounds, beta-elemene and totarol, were found in Greek samples in quite high amounts that are thought to have important biological properties.

No abstract

We aimed to determine the differences and similarities between Greek and Turkish propolis with respect to their chemical composition given that the two countries have many similarities in fl oral biodiversity. We observed that: a) Greek propolis is different from the European-type propolis, having a high terpene content; therefore we can definitely characterize it as a Mediterranean type; b) the Turkish propolis collected along the coast line of the Aegean Sea is similiar to the examined Greek propolis; c) the remaining Turkish samples, originating from the European part of Turkey, were found to be similiar to the Europeantype propolis, having a high fl avonoid content. Finally, especially two compounds, β-elemene and totarol, were found in Greek samples in quite high amounts that are thought to have important biological properties.

B u çalışma ile Türkiye propolisi için bitkisel bir köken olan Castanea sativa'nın anatomik ve palinolojik karakterlerinin araştırılması amaçlanmıştır. Bu kapsamda, Castanea sativa bitkisinin yaprak ve gövde enine kesitleri incelenmiştir. Mikroskobik inceleme ile yaprak enine kesitinde palizat ve sünger parenkiması, üst ve alt epidermis, sklerenkima ve iletim demetleri gözlenmiştir. Gövde enine kesitinde ise periderm, sklerenkimatik doku, sekonder ksilem, sekonder floem, vaskular kambiyum ve parenkimatik öz gözlenmiştir. Palinolojik analiz ile polenin radyal simetrik, izopolar ve trikolporat olduğu belirtilmiştir. Mikroskobik analizlerin yanında, Castanea sativa propolisinin kimyasal içeriği gaz kromatografisi ve kütle spektrometresi (GC-MS) cihazı ile analiz edilmiştir. Propolisin etanol özütünün kimyasal analizi ile alkoller, aldehidler, alifatik asit ve esterleri, karboksilik asit ve esterleri, sinamik asit ve esterleri, flavonoidler, hidrokarbonlar ve ketonlar grubuna ait bileşikler tespit edilmiştir. "Benzoik asit" bileşiği %7.88'lik oranla özütte en yüksek miktarda bulunan bileşiktir. Bu çalışmanın anatomik, palinolojik ve kimyasal analiz sonuçları Türkiye arıcılığı ve arı ürünleri ile ilgili ileriki çalışmalara ışık tutacaktır.

In this research, the melissopalynological analysis of honey samples collected from Kars city located in the East Anatolian Region of Turkey was conducted for geographical marking. Within this context, melissopalynological analyses of 100 honey samples determined by sampling method were collected from eight districts of Kars in Eastern Anatolia Region of Turkey were done, to determine the nectarous source plants of Kars honey. As a result of melisopalynological analyses carried out in 100 honey samples; pollens of the taxa belonging to Apiaceae, Asteraceae, Berberidaceae, Betulaceae, Brassicaceae, Boraginaceae, Campanulaceae, Caryophyllaceae, Chenopodiaceae, Cistaceae, Cyperaceae, Dipsacaceae, Ericaceae, Fabaceae, Iridaceae, Lamiaceae, Liliaceae, Malvaceae, Onagraceae, Papaveraceae, Plantaginaceae, Poaceae, Polygonaceae, Ranunculaceae, Rhamnaceae, Rosaceae, Rubiaceae, Rutaceae, Salicaceae and Scrophulariaceae families were detected at different rates. Almost in all of the honey samples, Lotus corniculatus (in 99 samples), Onobrychis radiata (in 99 samples), Trifolium nigrescens (in 88 samples) from Fabaceae family and pollens of Echium vulgaris (81 samples) and Myosotis lithoospermifolia (15 samples) taxa from the Boraginaceae family, were found in honey samples. Onobrychis radiata pollen was the most intensely observed one among these samples (in dominant, secondary, minor, trace amounts). The total number of pollens (TPN-10) in 10 grams of honey were also detected during the melissopalynological analyses. TPN-10 values minimum: 226, maximum: 481157 and mean: 31678 were detected and the pollen abundance of the honeys are classified as good category. Kars is an important province for beekeeping with floral variety. As a result of this study, the first step of the geographical marking studies of Kars' honey was completed. Keywords: Kars, Melissopalynology, Honey, TPN-10 Kars Balının Coğrafi İşaretlemesi İçin Melissopalinolojik Analiz ÖzetBu çalışmada, Türkiye'nin Doğu Anadolu Bölgesi'nde bulunan Kars İli'nde üretilen balların coğrafi işaretlenmesi için gerekli bir aşama olan melissopalinolojik analizleri yapılmıştır. Bu kapsamda sekiz ilçeden, örnekleme metoduna göre yapılan istatistiksel analizlerle tespit edilen 100 bal örneğinin mikroskobik analizleri gerçekleştirilerek Kars balına kaynaklık eden nektarlı bitkiler tespit edilmiştir. Bu amaçla melissopalinolojik analizleri yapılan 100 adet örnek balda; Apiaceae, Asteraceae, Berberidaceae, Betulaceae, Brassicaceae, Boraginaceae, Campanulaceae, Caryophyllaceae, Chenopodiaceae, Cistaceae, Cyperaceae, Dipsacaceae, Ericaceae, Fabaceae, Iridaceae, Lamiaceae, Liliaceae, Malvaceae, Onagraceae, Papaveraceae, Plantaginaceae, Poaceae, Polygonaceae, Ranunculaceae, Rhamnaceae, Rosaceae, Rubiaceae, Rutaceae, Salicaceae ve Scrophulariaceae familyalarına ait taksonların polenleri değişik oranlarda tespit edilmiştir. Fabaceae familyasından Lotus corniculatus (99 örnek), Onobrychis radiata (99 örnek), Trifolium nigrescens (88 örnek), Boraginaceae familyasından Echium vul...

No abstract