IGF-I and IGFALS play a vital stimulator role in skeletal growth, cell differentiation, metabolism, and other physiological processes. A total of 65 (male and female) animals were used in the study. Animals were measured for growth traits at birth weight, weaning weight, and weights at 6 months. The average daily gain (ADG) was calculated from birth to weaning (ADG1) and from birth to 6th month (ADG2). PCR-RFLP analysis was used to detect IGF-I polymorphism at the 5' regulatory region and IGFALS at Exon 1. Three genotypes (AA, AB and BB) were observed for IGF-I/BfoI locus with allele and genotype frequency 0.79(A) and 0.21(B); 0.71(AA), 0.15(AB) and 0.14(BB). Also, three genotypes (AA, AB and BB) were found for IGFALS/HinfI site with allele and genotype frequency as 0.22(A) and 0.78(B); 0.11(AA), 0.23(AB) and 0.66(BB). The genes were in agreement with Hardy-Weinberg equilibrium (p>0.05). Association analysis suggested that IGF-I and IGFALS significantly affected the growth traits (P<0.05). In terms of birth weight, The AA genotypes of IGF-I were higher than AB and BB. The AB genotype in terms of IGF-I had higher ADG2 compared with other genotypes. The AA genotype of the IGFALS gene was higher in terms of birth weight than other genotypes. In addition, the BB genotype was higher ADG1 than AA and BB. It is suggested that polymorphism of the IGF-I and IGFALS genes may be a potential molecular marker for growth traits in Hamdani sheep.
This study aims to identify the association between CAST/MspI and MSTN/HaeIII polymorphism with growth traits in Awassi sheep. A total of 129 (male and female) Awassi sheep were used in the study. Growth traits (Body weight BW, body length BL, chest depth CD, heart girth HG and withers height WH) were taken from animals at one year old. PCR-RFLP analysis was used to detect CAST polymorphism (Exon 1C/1D) and MSTN (Exon 3) genes. Three genotypes (MM, MN and NN) were observed for CAST/MspI polymorphism with allele and genotype frequency 0.78(M) and 0.22(N); 0.70(MM), 0.16(MN) and 0.14(NN). Two genotypes (MM and mm) were found for MSTN/HaeIII polymorphism with allele and genotype frequency as 0.10(M) and 0.90(N); 0.10(MM) and 0.90(mm). The genes were in agreement with Hardy-Weinberg equilibrium (p>0.05). The association analysis showed an association between CAST/MspI polymorphism and BW, BL, CD, HG and WH (P<0.05; P<0.10). The MM genotypes had the highest BW compared to MN and NN genotypes. Whereas the MN genotypes had the highest BL, CD, HG and WH compared to MM and NN genotypes. No association was found between MSTN/HaeIII polymorphism and growth traits.
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