Association between CAST and MSTN gene polymorphisms with growth traits in Awassi sheep

Abstract

This study aims to identify the association between CAST/MspI and MSTN/HaeIII polymorphism with growth traits in Awassi sheep. A total of 129 (male and female) Awassi sheep were used in the study. Growth traits (Body weight BW, body length BL, chest depth CD, heart girth HG and withers height WH) were taken from animals at one year old. PCR-RFLP analysis was used to detect CAST polymorphism (Exon 1C/1D) and MSTN (Exon 3) genes. Three genotypes (MM, MN and NN) were observed for CAST/MspI polymorphism with allele and genotype frequency 0.78(M) and 0.22(N); 0.70(MM), 0.16(MN) and 0.14(NN). Two genotypes (MM and mm) were found for MSTN/HaeIII polymorphism with allele and genotype frequency as 0.10(M) and 0.90(N); 0.10(MM) and 0.90(mm). The genes were in agreement with Hardy-Weinberg equilibrium (p>0.05). The association analysis showed an association between CAST/MspI polymorphism and BW, BL, CD, HG and WH (P<0.05; P<0.10). The MM genotypes had the highest BW compared to MN and NN genotypes. Whereas the MN genotypes had the highest BL, CD, HG and WH compared to MM and NN genotypes. No association was found between MSTN/HaeIII polymorphism and growth traits.

Determinatıon of the MSTN/HaeIII gene polymorphism in indigenous Morkaraman sheep

“…In studies, the m gene is seen at higher frequencies compared to the M gene (Soofy et al, 2009;Jamshidi et al, 2014;Akbari et al, 2015;Dimitrova et al, 2016;Lazar et al, 2016;Bayraktar, 2020;Haren et al, 2020), which is consistent with allele gene frequencies in our study.…”

“…Concerning different sheep breeds with regard to the MSTN gene, the Dalagh sheep breed (Azari et al, 2012), Zel sheep breed (Dehnavi et al, 2012), Karak- achan sheep breed (Bozhila-Sakova et al, 2016), and the Bulgarian Karnobat Merino sheep breed (Dimitrova et al, 2017) were found to be monomorphic. While the Mehreban sheep breed (Jamshidi et al, 2014), Kordi sheep breed (Akbari et al, 2015), Black-Headed Teleorman sheep breed (Lazar et al, 2016), Nilagiri sheep breed (Sahu et al, 2019), and Awassi sheep breed (Bayraktar, 2020) were found to be polymorphic with only two genotypes, the Sanjabi sheep breed (Soufy et al, 2009), Bulgarian Merino sheep breed (Dimitrova et al, 2016), and Batur sheep breed (Haren et al, 2020) were reported to be polymorphic with three genotypes. While studies indicating the MSTN gene polymorphism reported the mm genotype at a higher frequency than the MM genotype, the findings were consistent with our study.…”

Assessment of the association of the MOGAT1 and MOGAT3 gene with growth traits in different growth stages in Holstein calves

“…In contrast, fattening increases size caused by increased fatty tissue (Bayraktar and Shoshin, 2022b;Jo et al, 2009). With the development of molecular techniques, it has become possible to identify genes that directly or indirectly affect the growth characteristics of animals (Bayraktar and Shoshin, 2022c;Park et al, 2018). Acyl coenzyme A, monoacylglycerol acyltransferase (MOGAT), also known as MGAT, is a vital enzyme that plays an active role in catalyzing the synthesis of triacylglycerol (TAG) and diacylglycerol (DAG; Lee and Kim, 2017;Cao et al, 2003;Hall et al, 2012).…”