Heat shock proteins (HSPs) are chaperones with highly conservative primary structure, necessary in the processes of protein folding to the most energetically advantageous conformation and maintaining their stability. HSPs perform a number of important functions in various cellular processes and are capable of modulating pathophysiological conditions at the cellular and systemic levels. An example is the high level of HSP expression in neoplastic tissues, which disrupts the apoptosis of transformed cells and promotes the processes of proliferation, invasion, and metastasis. In addition, an increasing amount of information is appearing about the participation of HSPs in the formation of multidrug resistance.This paper provides a review of the current state of research on the fundamental importance as well as the diagnostic and prognostic role of various classes of HSP in cancer treatment. It presents the prospects for using HSPs as biological markers of disease progression and targets in various cancer treatment strategies. However, the need for additional research is quite high. Only numerous joint efforts of research groups will allow the effective use of HSPs as a tool to combat cancer.

Determining the value of a half-effective or half-life concentration or dose of toxicant is the main purpose of acute toxicity studies, and this is also the most commonly used value in the toxicity characteristics of substances. By conducting tests that meet the criteria and requirements for the determination of acute toxicity, due to the use of appropriate mathematical tools and concentrations resulting in complete lethal effects in the studied groups, considerably more important values can be achieved, which give a possibility for the analysis of the entire process’s dynamics, as well as determining the threshold values of the effect time and toxicant concentration. This was the purpose of our research, in which the research species were Daphnia magna and Cypris pubera. The effect of the conducted research allowed to determine and compare the two toxicants: ammonium and copper(II) ions by it’s: concentration limit values (Cth), internal toxicity of the receptor-ligand complex (α), apparent, constant disintegration of this complex (Kapp) and different time values of the effect (Tt, Tin, MLT), which, along with concentration, is equally important determinant of the development of a toxic effect.

As a heavy metal, cadmium has strongly toxic effects on plants and can induce oxidative stress. It is absorbed by the roots and transported to the stems and leaves. The aim of the study was to evaluate the effect of various concentrations of cadmium on the metabolic activity of Beta vulgaris and assess the dependence of these processes on the content of metal in the plants. To demonstrate the effect of cadmium on metabolism, protein and photosynthetic pigment content, lipid peroxidation, and the activity of enzymes specific for oxidative stress in roots and shoots were measured. Seeds of B. vulgaris were treated with different concentrations of Cd supplied via a CdCl2 solution: 0 (control), 200, 300 and 400 mg/dm3. Results of the present study revealed increased GPOX activity as cadmium concentration rose, while SOD activity was stimulated by a low Cd concentration (200 mg/dm3) and reduced by high levels of Cd. Based on the present findings, it can be concluded that GPOX in B. vulgaris played a more important role in ROS scavenging than SOD did and was able to reduce the level of lipid peroxidation in plants. Cadmium, in the concentration range used, did not show any significant effect on protein or photosynthetic pigment content.

Changes in the barrier mechanisms in the eye should determine the rational route for the administration and dosage of each drug in the treatment of traumatic injuries and other pathologies. The aim of this study was to examine the efficacy of intra-arterial delivery of 14C-riboflavin (as an “indicator”) and compare it with intravenous and intramuscular administration in an animal model of chemical eye burn. 14C-riboflavin (14C-I) was administered by intra-arterial (carotid artery), intravenous (femoral vein) and intramuscular (femoral muscle) routes. The total radioactivity was determined over 2 h in the plasma and structures of the rabbit’s eyes using a scintillation counter. The results of the study show that intravascular administration of 14C-I gives significantly higher concentrations of total radioactivity in the blood and is accompanied by a significant increase in the permeability of the blood-barrier and barrier in eyes suffering from burns. The highest concentration in the plasma and aqueous humour of the anterior chamber of the eye was observed during the first hour with the intra-arterial route of administration of 14C-I in either burnt and unburnt eyes. The distribution of total radioactivity in the structures of the eye over the 2 h of the experiment showed a higher level of the drug under intra-arterial administered in the uveal regions, namely: the iris, ciliary body, choroid, retina and also the sclera and cornea. This experimental model shows that intra-arterial administration can increase the bioavailability of a drug to the structures of the eye within a short period of time.

Introduction The low serum concentration of the anti-tuberculosis (TB) drugs in TB patients is observed quite often, but the significance of this phenomenon remains controversial. Aim The aim of the presented research was to clarify the association between isoniazid concentration and TB-treatment outcomes. Material and methods Blood samples were obtained from 86 patients with newly diagnosed pulmonary TB at Odessa Regional TB Hospital in 2015. The level of isoniazid in blood was measured 2 h, 4 h, 6 h, and 24 h after the administration of isoniazid. The medical records of the enrolled patients at the beginning and at the end of the in-patient treatment, and the activity of the biochemical indices were considered. Results and discussion At the end of the in-patient treatment in patients with a serum isoniazid concentration of more than 2 µg/mL (HIC), 4 h after the drug administration, the resorption of TB infiltrates in lungs occurred 1.3 times more frequently than in patients with an isoniazid concentration of less than 2 µg/mL (LIC). According to the culture method, the smear conversion occurred 3.1 times more often in patients with HIC than in the LIC ones. A positive correlation was found between the isoniazid serum level with the bilirubin level, thymol probe, and the AsAT activity, which proved the higher risk of hepatotoxicity in patients with HIC. Conclusions Thus, the measurement of the isoniazid concentration after 4 h of isoniazid administration can predict the outcome of the TB-treatment. It is postulated that the recommended concentration of isoniazid in blood 4 h after its administration > 2 µg/mL.