A method of antibody elution from red blood cells using xylene is described. It can be completed in 15 minutes after cell washing and requires no special equipment. For warm antibodies, the eluates prepared were more potent than those prepared by Rubin's ether method. It was as effective as Landsteiner and Miller's heat elution method in investigations of ABO hemolytic disease of the newborn. The clinical application of this method in the investigation of hemolytic disease of the newborn, delayed transfusion reaction and autoimmune hemolytic anemia was described.
Fifty patients with cervical incompetence were randomised to have cervical cerclage either as inpatients, spending 3 days in hospital post-procedure on supervised bed rest or as outpatients spending the time at home on bed rest. Both groups had a clinical diagnosis of cervical incompetence and both had either McDonald or Shirodkar cerclage with mersilene tape. Both groups were given salbutamol tablets for tocolysis, postoperatively. There were no significant difference in the demographic variables between the groups such as previous cerclage, gestational age at insertion, parity and gestational age at delivery. There were also no significant differences in early complications such as bleeding. Most late complications were also not different, including the spontaneous abortion rate, premature rupture of membranes, cervical dystocia and preterm delivery. However, more patients in the outpatient group had premature contractions (26.1% vs. 4.3% P=0.0479). More patients in the inpatient group had a delivery of a live neonate, 86.9% vs. 78.3%, but the difference was not statistically significant. In conclusion, out patient cerclage appears to be a valid option, the higher rate of premature contraction in this group is not a cause for concern in view of the similar mean gestational age at delivery.
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