Peste‐des‐petits‐ruminants (PPR) and Goat pox (GTP) are two devastating and economically important transboundary animal diseases of small ruminants in Africa and Asia that have been difficult to control. This study however, investigated an outbreak of PPR and GTP in a mixed flock of indigenous sheep and goats in Kanam, North Central Nigeria. A total of nine sera and seven tissues (lungs, spleen, scab and skin) samples were collected and analysed in the laboratory using competitive enzyme linked immunosorbent assay (cELISA) for PPR antibodies and polymerase chain reaction (PCR) for detection of PPR virus (PPRV) and GTP virus (GTPV). Gene fragments of the nucleoprotein of PPRV and the G‐protein‐coupled chemokine receptor (GPCR) of GTPV were amplified and sequenced to confirm the presence of the causative viruses. Serologically, antibodies to PPRV were detected in all (9/9) sera collected. GTPV and PPRV was detected in corresponding samples (42.8% n = 3/7) of the scab/skin samples collected by both PCR and RT‐PCR technique. The phylogenetic analysis of PPRV revealed that the virus belongs to lineage IV and clustered with viruses from Gabon and Cameroon. Similarly, the GTPV also clustered with other sequences from Burkina Faso and Yemen. The positive cELISA, RT‐PCR and PCR results from samples collected from the same animals confirmed co‐infection of PPR and GTP in this mixed flock of sheep and goats. This is the first report of concurrent infection of PPR and GTP in mixed flock of sheep and goats in Nigeria. Our findings underscore the need for farmers to vaccinate their flock to control spread and economic losses as result of these diseases.

Coccidiosis is a disease of economic importance in poultry causing morbidity and mortality. Reports show that Azadirachta indica and Khaya senegalensis have been used individually in the treatment of avian coccidiosis. We thus investigated the efficacy and safety of the combined aqueous extracts of these plants for the treatment of experimentally induced coccidiosis in broiler chickens using oocyst count, oxidative stress biomarkers, serum biochemistry, histology, and haematological parameters. The phytochemical screening revealed the presence of tannins, saponins, cardiac glycosides, and steroids in both extracts. In addition, alkaloids and flavonoids were present in Azadirachta indica. There was significant (p < 0.05) dose dependent decrease in oocyst count across the treatment groups with 400 mg/kg of the combined extract being the most efficacious dose. Immunomodulatory and erythropoietic activity was observed. There were decreased intestinal lesions and enhanced antioxidant activity across the treatment groups compared to the negative control. Administration of the combined extract did not cause damage to the liver as ALT, AST, and ALP levels were significantly reduced in the uninfected chickens treated with the extracts compared to control suggesting safety at the doses used. The combined aqueous extracts of K. senegalensis stem bark and Azadirachta indica leaves were ameliorative in chickens infected with coccidiosis.

Commercial layer-type, pullet, cockerel, and broiler chicken flocks infected with highly pathogenic avian influenza (HPAI) H5N1 in Nigeria between 2006 and 2008 were investigated for morbidity, mortality, and pathology. Of the one hundred and fifty-three (153) farms confirmed with HPAI infection, one hundred and twenty-seven (127) were layer-type farms, nine (9) were pullet and broiler farms each, and eight (8) were cockerel rearing farms. This study revealed the morbidity and mortality of a total of 939,620 commercial layer chickens, 16,421 pullets, 3,109 cockerels, and 6,433 broilers. Mortality rates were 11.11% in commercial layers, 26.84% in pullets, 45.51% in cockerels, and 73.92% in broilers in a total of eighteen (18) states and the Federal Capital Territory, Abuja. A total of 316 carcasses were examined of which 248 were commercial layer, 25 were pullet, 14 were cockerel, and 29 were broiler. Main clinical and pathologic findings were observed in the nervous, circulatory, respiratory, integumentary, musculoskeletal, hemopoietic, gastrointestinal, and reproductive systems and, occasionally, lesions were generally nonspecific and multisystemic. Lesions occurred more frequently, severely, and in most of the carcasses examined, irrespective of chicken type.

Background. In early 2010, outbreaks of lead poisoning due to artisanal gold mining in villages in the northwest Nigerian state of Zamfara have resulted in the death of hundreds of children < 5 years old. There have also been unconfirmed reports of high mortality of geese within these villages. Objectives. To report a case of lead poisoning in three domestic free-range chickens found in one of the affected communities where illegal small-scale gold mining activities take place. Methods. Three free-range domestic chickens were presented during a field investigation in one of the villages. The birds were observed to be emaciated, weak, showing nervous manifestations and moribund. Results. Tissue extracts of liver, spleen and intestines were negative for Newcastle viral antigens, while cultures of liver and spleen biopsy were positive for Escherichia coli . Histopathological lesions were observed in the kidney, proventriculus and brain. Concentrations of lead in the tissues ranged between 7.5 mg/kg and 120.5 mg/kg wet weight, and the potential daily intake of lead in the tissues were estimated at 34.06–200.15 μg/day/kg body weight with an average of 118.37 μg/day/kg body weight. Conclusions. The results of the present study suggest probable risk to human health due to the consumption of chicken contaminated by lead in the affected villages. Poisoning in animal populations may serve as a sentinel to assess the extent of environmental contamination and human health problems related to lead. Ethics Approval. Protocols were approved and performed in accordance with relevant local guidelines and regulations as set by the Animal Care and Use Committee of the National Veterinary Research Institute, Vom, Nigeria. Competing Interests. The authors declare no competing financial interests.

Background: Parquetina nigrescens is among the evergreen plants native to West Africa. It is used in the management of various ailments including anemia, fever, asthma and diabetes. This study evaluated the antidiabetic and antihyperlipidemic effect of Parquetina nigrescens in streptozotocin-nicotinamide-induced type 2 diabetic rats. Methods: Type 2 diabetes mellitus was induced in overnight fasted rats with a single intraperitoneal injection of streptozotocin (60 mg/kg), followed by the administration of nicotinamide (120 mg/kg) after an interval of 15 min. Diabetic rats were orally administered with; 200, 400 and 800 mg/kg of aqueous extract of Parquetina nigrescens (AEPN), metformin (180 mg/kg) and glibenclamide (1 mg/kg) for two weeks. The effect of treatments on fasting blood glucose, serum insulin, leptin, adiponectin, homa-ir, lipid profile, body weight, pancreatic antioxidants parameters, hepatic glycogen content, glucose-6-phosphate activity, α-amylase inhibition, α-glucosidase inhibition, lipase inhibition and histology of the organs were evaluated. Results: Data from this study showed that treatment with AEPN produced a significant reduction (p < 0.05) in fasting blood glucose, glucose-6-phosphatase activity, serum lipase, total triglyceride, total cholesterol, lowdensity lipoproteins, very low-density lipoprotein, atherogenic index, coronary risk index, pancreatic α-amylase, α-glucosidase and lipase activities. Treatment with AEPN also produced a significant (p < 0.05) increase in; glucose tolerance, glycogen content, leptin, adiponectin and pancreatic antioxidants (glutathione, superoxide dismutase, catalase and high-density lipoproteins). The histology of the organ showed regeneration of the pancreatic tissue after treatment with AEPN. Conclusions: This study showed that AEPN exhibited antidiabetic and antihyperlipidemic activity in streptozotocin-nicotinamide-induced type 2 diabetic rats.

Infection of small ruminants with peste des petits ruminants virus (PPRV) and goatpox virus (GTPV) are endemic and can have devastating economic consequences in Asia and Africa. Co-infection with these viruses have recently been reported in goats and sheep in Nigeria. In this study, we evaluated samples from the lips of a red Sokoto goat, and describe co-infection of keratinocytes with PPRV and GTPV using histopathology and transmission electron microscopy. Eosinophilic cytoplasmic inclusion bodies were identified histologically, and ultrastructural analysis revealed numerous large cytoplasmic viral factories containing poxvirus particles and varying sizes of smaller cytoplasmic inclusions composed of PPRV nucleocapsids. These histopathological and ultrastructural findings show concurrent infection with the 2 viruses for the first time as well as the detection of PPRV particles in epithelial cells of the mucocutaneous junction of the lip.

A dairy farm in Keffi, Nasarawa State, North Central Nigeria reported cases of skin infections in cattle suggestive of lumpy skin disease (LSD) in the years 2014 and 2016. During the 2016 outbreak, skin biopsies were collected for laboratory investigation using histopathology and polymerase chain reaction (PCR). The samples were subjected to PCR using two sets of primers which amplified the G-protein-coupled chemokine receptor (GPCR) gene and RNA polymerase subunit gene (RPO30) of the LSD virus (LSDV). Clinical signs observed in the two outbreaks were similar and included ocular and nasal discharges, nodular skin lesion, and pyrexia and reduced milk production. Morbidity rate was 6.25% (25/400) in 2014 and the mortality rate was 2.5% (10/400). Furthermore, the second outbreak in 2016 had morbidity and mortality rates of 5% (20/400) and 0.5 % (2/400) respectively. From animals that died in the 2014 outbreak, necropsy findings included nodular lesions on the skin, muzzle, in the buccal cavity and nasal mucosa. Also, congested and oedematous lungs and enlarged lymph nodes were observed. The nodular skin lesions observed during the 2016 outbreak were larger and more numerous in the Sokoto Gudali, while the Holstein Friesian cross had smaller gross skin nodules with acute to sub-acute histopathologic changes. The histopathological examination of the skin biopsy of the HF was consistent with LSD viral lesions, while PCR detected the RPO30 and GPCR gene fragments of LSDV.

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