Background: Cuttlefish bone Xenograft, calcium phosphate (CaP)-based biomaterial graft, offers an alternative and has been accepted for osteoconductive and probable osteo-inductive attributes. This study aims to compare the bone healing potential between the bovine-derived (BHA) and cuttlefish bone-derived (CHA).Materials and Methods: The study compared osteoblast cell proliferation of 27 New Zealand rabbits in 2.5 mm bone defect made in the femoral bone. The samples were divided into three groups, which were control, BHA and CHA group. The chemical and physical characteristics of BHA and CHA were determined for the content of hydroxyapatite by FourierTransform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD), then tested by Scanning Electron Microscopy (SEM) to evaluate the porosity. In the end of the second week, histopathologic and immunohistochemistry examinations were performed to evaluate the amount of osteoblast and osteocalcin expression.Results: The FTIR, XRD and SEM analysis showed both BHA and CHA samples were hydroxyapatite according to Joint Committee on Powder Diffraction Standards (JCPDS). The CHA was significantly higher (297.22±19.772) compared to BHA (258.22±30.926) and control (131.67±34.213). Osteocalcin expression in CHA (7.82±2.230) compared to BHA (6.09±3.724) and control (4.07±3.606), was not significant (p>0.05). Conclusion: CHA group has the highest osteoblast cell proliferation and osteocalcin expression, meaning has a good potential as future source of bone graft.Keywords: cuttlefish bone, bovine, bone graft, osteoblast cell
Background: Tuberculous spondylitis or spinal tuberculosis, which is also known as Pott's disease of the spine is a disease that occurs throughout the world. This disease affects the spine caused by the bacteria Mycobacterium tuberculosis (TB). MMP-9 is a proteolytic enzyme that is thought to play a role in the progressive degradation of extracellular matrix in physiological and pathophysiological processes involving remodeling of spinal tissue. This study wanted to examine the role of doxycycline as an MMP-9 inhibitor in a rabbit model of tuberculous spondylitis infection. Methods: This type of research is an experimental study using a randomized controlled design method with a One Group Posttest Only design. We conducted this research from March -June 2020 at the Animal Hospital of the Bogor Agricultural University (IPB). The population of this study was adult New Zealand rabbits (Oryctolagus cuniculus) weighing 1.8-2 kg with 40 rabbits divided into 12 groups. Results: In this study, there was a significant difference between the delta values between the inoculation duration groups, both in the treatment group without doxycycline, doxycycline 1 mg/kgBW, and doxycycline 5 mg/kgBW. Regarding the effect of doxycycline administration, only Group D (decrease) and Group H (increase) showed a significant effect of doxycycline administration on MMP-9 levels in experimental animals with TB spondylitis. Conclusion: MMP-9 will still increase even though doxycycline intervention has been given considering that the occurrence of a chronic process in which inflammatory factors will continue to increase even though antibiotic therapy has been given to suppress the number of these inflammatory factors.
Background: The blood supply of a bone can be decreased due to the use of an implant, leading to wound-bed bacterial colonization and the development of the SSI. The principle of vacuum-assisted closure (VAC) is to use a negative pressure environment in the wound to promote increased wound healing. The case shows that VAC application and muscle flap therapy provide the definitive healing of infected wounds after ORIF. Case Report: A 45 years-old male presented with a close comminuted proximal third right tibia fracture and tense skin. The fracture was openly reduced and internally fixated with plate fixation and a skin flap on the fracture area. Still, the wound could not be closed due to difficulty covering the wound, so a counter incision was made. After four days, the patient developed necrosis in the post-operation wound and subsequently underwent debridement and muscle flap with VAC application. Discussion: SSI can be treated by applicating of appropriate antibiotic and surgical procedures. In this case, the infection was treated using third-generation cephalosporin antibiotic, debridement, dissection, and re-elevation of gastrocnemius muscle flap covering the exposed plate area in inferior, split skin graft above the muscle flap and VAC above skin graft with 75 mmHg pressure. Conclusion: Debridement with muscle flap and application of VAC in the deep infected wound of open reduction and internal fixation was found to be successful methods in minimizing complications and obtaining an optimal outcome. This finding was associated with accompanying morbidities or a good wound healing process.
Background: Osteochondroma is a benign bone tumor that most cases are asymptomatic. The symptoms may occur as a complication of osteochondroma, which is rarely happened. Surgical intervention is needed to treat the complications.Case: A 20-year old male football athlete presented with pain on his left thigh's lump after he fell during his training. Mass and tenderness on the medial side of the left distal femur were found. Plain X-Ray showed an irregular mass on the left distal femur's surface with a fracture through the stalk of pedunculated osteochondroma. The patient underwent total excision of the osteochondroma.Discussion: The fracture of the osteochondroma often occurs in teenagers because they are more active physically. It happens due to direct or indirect injury. The treatment of this fracture is controversial between observation or surgical excision. In this case report, the patient could demonstrate full weight-bearing right after the surgery.Conclusion: Fracture as a complication in osteochondroma often happens to teenagers, and surgery proved to give a good outcome in this case.
Background: Neck femur fracture has a poor prognosis due to its anatomical structure. Fractures that occur in the elderly often have a heavy comorbid so that the mortality rate in 1 year in this case reaches 30-50%. The techniques that have been developed at this time are not satisfactory results with non-union rate of about 30%.Purpose: Assessing the long-term effectiveness of fibular auto strut graft techniques for neck femoral fracture therapy.Methods: The study was conducted retrospectively using primary data in the form of clinical evaluation and secondary data in the form of medical record data and questionnaires of neck femur fracture patients who performed non-vascularized autofibular strutgraft therapy in the period January 2005 to August 2015 in operating room IRD RSUD Dr. Soetomo Surabaya. Patients were evaluated with Harris Hip Score.Result: Medical record data shows that surgery was performed in January 2005 until August 2015 in the IRD hospital operating room. Soetomo Surabaya with 15 patients that fit criteris inclusion. The final result of Harris Hip Score is excellent in 66.66% of patients, good in 20% of patients, and poor on 13.33% of patients. It can be seen that in large part, the technique of autofibular strutgraft (Surabaya Technique) gives good results to the sufferer.Conclusion: Autofibular strutgraft and reinforcement with cancellous lag screw on neck femur fracture can generally provide good functional results in long term evaluation. From the assessment of functional aspects, abnormal anatomical conditions, and Range of Motion (ROM), obtained satisfactory results. This supports autofibular strutgraft as a neck femur fracture therapy as a major therapeutic option at a young age.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.