A Comparison of Osteoblast Cell Proliferation and Osteocalcin Expression in Cuttlefish Bone and Bovine Bone Xenograft

Irianto, Komang Agung; Pribadi, Ameria; Irsyam, OK Ilham Abdullah; Kloping, Yudhistira Pradnyan; Sindrawati, Oen

doi:10.21705/mcbs.v3i2.58

Cited by 3 publications

(7 citation statements)

References 20 publications

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“…19 Other studies found that the cuttlefish bonegraft has the potency to induce osteoblast differentiation and proliferation. 7,20,21 This circumstance is postulated because of the presence of Mg²⁺ on the material, which was proven to promote osteogenesis. 22,23 Thus, cuttlefish bone powder poses low toxicity and ability to promote osteogenesis, prompting its possible application as bone graft material.…”

Section: Discussionmentioning

confidence: 99%

“…The acute systemic toxicity examination in this study for PerOssal®, cuttlefish bone, and control groups showed minor acute systemic toxicity reaction and pyrogenicity, which is consistent with the previous experiment with rat fracture model. 6,7,19 Dogan and Okumus 8 reported that cuttlefish bone xenograft did not affect the physiological measurement of the NZ rabbit after 24 weeks of implantation, allowing the cuttlefish bone to become bone xenograft potential material. They also showed no increase in inflammatory cells histologically; thus, the graft does not cause inflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

“…The 0.6 M NH₄H₂PO₄ solution was prepared by dissolving 69 g of the compound in 1 liter of distilled water. 7,16 The hydrothermal reaction to produce hydroxyapatite (HA) was as follows: 10 CaCO₃ + 6 NH₄H₂PO₄ + 2H₂O → Ca₁₀(PO₄) 6(OH)₂ + 3 (NH₄) 2CO₃ + 7 H₂CO₃. The reaction was carried out by mixing two previous solutions using a magnetic stirrer for 30 min.…”

Section: Preparation Of Cuttlefish Bone Xenograftmentioning

confidence: 99%

“…The morphology and the ratio Ca/PO₄ were quantified using scanning electron microscope-energy dispersive X-ray spectroscopy. 7,17 The synthetic bone graft was PerOssal® (AAP biomaterials GmbH, Germany; product number: 42000000-AKS-004699091). It is in powder form and ready to use.…”

Section: Preparation Of Cuttlefish Bone Xenograftmentioning

confidence: 99%

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Cytotoxic effect of natural cuttlefish bone xenograft: an in vitro and in vivo study

Irianto

Limbong

2020

Med J Indones

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BACKGROUND Commercialized synthetic bone grafts are commonly used to replace the bone defect. Cuttlefish bone is naturally available and widely studied, but the specific cytotoxicity test has not been conducted. This study aimed to evaluate the cytotoxicity of the xenograft compared to commercial grafts. METHODS We performed an in vitro test evaluating the viability of human mesenchymal stem cells (hMSCs) when cultured for 48 hours with the tested materials (cuttlefish bone graft and fabricated PerOssal®). The trypsinized mitochondrial activity of the viable hMSC was assayed based on colorimetry of the formazan color change. The tested material was considered nontoxic if >70% of the hMSCs were viable. The in vivo cytotoxic effect was evaluated by implanting the graft material in the femoral muscle of New Zealand (NZ) white rabbits. Nine rabbits were used in each test (cuttlefish bone, PerOssal®, and NaCl 0.9%). The systemic acute pyrogenic effect was evaluated based on 72 hours body weight changes and rectal temperature changes every 30 min in the first 3 hours and 72 hours post-implantation. RESULTS The mean percentage of hMSC viability when cultured with cuttlefish bone graft and PerOssal® was comparable (93.47% and 105.37%, respectively, p = 0.240). The in vivo cytotoxicity on NZ rabbit was similar between all tested materials, as shown by the minor changes in body weight (<10% body weight, p = 0.391) and rectal temperature (<0.5°C, p = 0.127). CONCLUSIONS Cuttlefish bone xenograft and fabricated PerOssal® have a similar non-cytotoxic effects on hMSCs and non-pyrogenic systemic effects on rabbits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Cuttlefish Bone Xenograftmentioning

confidence: 99%

Section: Preparation Of Cuttlefish Bone Xenograftmentioning

confidence: 99%

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Cytotoxic effect of natural cuttlefish bone xenograft: an in vitro and in vivo study

Irianto

Limbong

2020

Med J Indones

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“…Levels of osteocalcin and osteoblast cells are components used as markers of the bone healing process. 8 , 9 Sari et al . 10 studied the use of seashells and sea cucumber on the femoral defects of Wistar rats and found significant differences in the number of osteoblasts and osteoclasts between groups.…”

Section: Introductionmentioning

confidence: 99%

Application of green mussel (Perna viridis) shells hydroxyapatite on osteocalcin levels and osteoblast cells in rabbit femur bone defect

Putra,

Widyastiti,

Budijitno

et al. 2023

Annals of Medicine &Amp; Surgery

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Background: Bovine hydroxyapatite (HA) used for bone grafts is relatively expensive, necessitating the development of alternative sources. Alternative HA materials derived from green mussel shells with smaller molecular sizes are inexpensive and abundantly available throughout Indonesian waters. The purpose of this study is to investigate the effect of green mussel shells HA on bone healing. Methods: This post-test-only experimental research used male rabbits with femoral defects divided into three groups randomly: K (no treatment), P1 (bovine HA treatment), and P2 (green mussel shell HA treatment). The osteocalcin level was assessed biochemically while osteoblast cells were histopathologically at the second, fourth, and sixth weeks. Statistic tests were used to assess differences between groups and periods with statistical significance P<0.05. Results: Nine rabbits in each group showed significant differences between groups K, P1, and P2 in term osteocalcin levels at week 2 (2.60, 4.53±0.12, 4.47±0.23; P=0.046), week 4 (5.13±0.12, 8.53±0.12, 7.47±0.12; P=0.025), and week 6 (8.20, 11.93±0.23, 10.93±0.31, P=0.023), while in term osteoblast cells only at week 6 (16.33±3.46, 26.10±3.52, 30.40±3.29; P=0.006). The osteocalcin level and osteoblast increased significantly between groups K and P1/P2 from the initial trial until the last week. Osteoblast cells in the groups P1/P2 increased significantly, especially at week 6. Conclusion: Green mussel shell HA has the biochemical effectiveness of osteocalcin and can increase osteoblast cells comparable to bovine HA, which can enhance bone healing.

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Effects of Aging on Osteosynthesis at Bone–Implant Interfaces

Pius,

Toya,

Gao

et al. 2023

Biomolecules

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Joint replacement is a common surgery and is predominantly utilized for treatment of osteoarthritis in the aging population. The longevity of many of these implants depends on bony ingrowth. Here, we provide an overview of current techniques in osteogenesis (inducing bone growth onto an implant), which is affected by aging and inflammation. In this review we cover the biologic underpinnings of these processes as well as the clinical applications. Overall, aging has a significant effect at the cellular and macroscopic level that impacts osteosynthesis at bone-metal interfaces after joint arthroplasty; potential solutions include targeting prolonged inflammation, preventing microbial adhesion, and enhancing osteoinductive and osteoconductive properties.

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A Comparison of Osteoblast Cell Proliferation and Osteocalcin Expression in Cuttlefish Bone and Bovine Bone Xenograft

Cited by 3 publications

References 20 publications

Cytotoxic effect of natural cuttlefish bone xenograft: an <em> in vitro </em> and <em> in vivo </em> study

Cytotoxic effect of natural cuttlefish bone xenograft: an <em> in vitro </em> and <em> in vivo </em> study

Application of green mussel (Perna viridis) shells hydroxyapatite on osteocalcin levels and osteoblast cells in rabbit femur bone defect

Effects of Aging on Osteosynthesis at Bone–Implant Interfaces

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