Water quality is made up of physical, chemical and biological factors which influence the use of water for domestic purposes. Industrial and municipal solid and liquid wastes are being continuously leached into water reservoirs, thereby affecting its potability for domestic use. In this study, the physicochemical, bacteriological and parasitological evaluation of selected well water samples in Awka and its environment were evaluated during wet season. Standard methods for physicochemical, bacteriological and parasitological analyses were employed. Physicochemical analysis was done using standard analytical methods; bacteriological analysis was determined by dilution and membrane filtration techniques. Parasitological analysis was done using the centrifugation method. The result showed that 86.67% of the pH values were acidic,  6.67% of nitrate, 33.33% of phosphate, 20% of cadmium, 73.33% of lead, 26.67% of arsenic, 20% of iron, 100% of bacteria and parasites exceeded the WHO maximum containment level goal for domestic water while other parameters were within WHO standards for domestic water. The most polluted of all the well water samples is Aka well water while the least polluted is Emeka and Aqua well waters. S. typhi had the highest frequency of isolation (25.45%) while Bacillus subtilis had the least (1.56%). Diplostomum parasite had the highest frequency of isolation (42.86%) while Ichthyobodo and Chilodonella had the least (28.57% respectively). Since some of the physicochemical, bacteriological and parasitological parameters had values above World Health Organization admissible limits; governments, health and environmental experts must rectify (through water treatments and better sanitary practices) and enlighten the residents to prevent epidemics.

Water as excellent natural resource is meant to be of good quality to prevent the outbreak of water-borne diseases. The physical, chemical and biological qualities of water constitute groundwater quality. Water of poor physicochemical quality may have adverse effects on human health and the economy. The physicochemical evaluation of fifteen borehole waters in mile 50 Abakaliki was carried out during the rainy and dry seasons to determine their suitability for drinking using standard analytical methods. During the rainy season, the temperature was 28-30°C; pH, 6.63-8.51; dissolved solids, 1.04-17.01 mg/l; total suspended solids, 0.09-0.98 mg/l; total solids, 1.14-17.99 mg/l; electrical conductivity, 107-328 us/cm; turbidity, 1.27NTU-2.60 NTU total alkalinity, 27.68-82.23 mg/l; total hardness, 70.20-150.84 mg/l; total chloride, 67.30-124.14 mg/l; calcium hardness, 24.50-53.58 mg/l; magnesium hardness, 39.40-97.26 mg/l; sulphate, 30.03-61.88 mg/l; phosphate, 0.25-6.71 mg/l; potassium, 0.00-8.04 mg/l; nitrate, 1.16-8.03 mg/l; iron, 0.00-0.26 mg/l; lead, 0.00-0.05 mg/l; cadmium, 0.00-0.04 mg/l; copper, 0.00-0.23mg/l; chromium, 0.00-0.05 mg/l and zinc, 0.07-2.15 mg/l. During the dry season the temperature was 27-29°C; pH, 6.40-7.75; electrical conductivity, 24-149 us/cm; dissolved solids, 0.10-2.03 mg/l; total suspended solids, 0.02-0.29 mg/l; total solids, 0.13-2.64 mg/l; turbidity, 0.61NTU-1.90 total alkalinity, 19.96-55.97 mg/l; total hardness, 49.61-82.35 mg/l; total chloride, 26.31-80.72 mg/l; calcium hardness, 5.63-29.30 mg/l; magnesium hardness, 30.54-67.30 mg/l; sulphate, 13.88-39.18 mg/l; phosphate, 0.07-3.18 mg/l; potassium, 0.00-4.73 mg/l; nitrate, 0.44-4.95 mg/l; iron, 0.00-0.16 mg/l; lead, 0.00-0.02 mg/l; cadmium, 0.00-0.02 mg/l ; copper, 0.00-0.15 mg/l; chromium, 0.00-0.03 mg/l and zinc, 0.02-0.64 mg/l during the dry season. From the average 7% of cadmium and 33% of lead in the water samples were above the NIS required limits of 0.003 and 0.01mg/l respectively. Generally, the water from the borehole was of poor physicochemical quality and must be treated adequately before being used by humans.

Medicinal plants are used by almost 80% of the world’s population for their basic health care because of their low cost and ease in availability. In the last few decades, many bacteria have continued to show increasing resistance against current antibiotics. Aim: In this study, phytochemical screening and antibacterial effects of conventional antibiotics, garlic and ginger on test isolates from fish pond water samples were evaluated between May-November, 2019. Methods: Standard methods for phytochemical screening and antibacterial analysis were employed. Results: The results showed that amongst the antibiotics used for susceptibility test, Amoxicilin (30 µg) was mostly resisted by all the bacterial isolates except Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhi while erythromycin (10 µg) was unable to inhibit Bacillus subtilis. Ciprofloxacin (10 µg) and pefloxacin (10 µg) inhibited the growth of all the isolates except Pseudomonas aeruginosa. The test isolates showed variable susceptibility to the garlic and ginger extracts (ethanol, methanol and hot water). The extracts inhibited the isolates in descending order; ginger ethanol > ginger methanol > garlic methanol > ginger hot water > garlic ethanol > garlic hot water. Vibrio parahaemolyticus, Vibrio cholerae and Staphylococcus aureus showed little resistant to the extracts while these extracts showed better activity on Klebsiella pneumoniae and Proteus mirabilis. Synergistic effect of garlic and ginger (500mg/ml) inhibited the growth of all the isolates with ethanol extracts having the highest zone diameter (29 mm) on Klebsiella pneumoniae and Proteus mirabilis while hot water extracts had the least zone of inhibition (18 mm) on Acinetobacter calcoaceticus and Vibrio parahaemolyticus. The minimum inhibitory and bactericidal concentration for ethanol, methanol and hot water extracts ranged from 31.25mg/ml to 62.5mg/ml and 62.5mg/ml to 125mg/ml respectively. Conclusion: The outcomes of susceptibility experiment depicted that ethanol and methanol extracts of garlic and ginger (each alone and in combination) showed more inhibitory effect than aqueous extracts and also the combination of ethanol, methanol and aqueous extracts resulted in inhibitory effect greater than each of the extracts when used singly. The use of ginger and garlic for control of fish pathogens appears to be justified.

Background: The people of Awka urban show an increasing trend of using well water resulting to unreliable and quality-compromised water supply. Aim: Effects of depth and seasons on the physicochemical and bacteriological quality of selected well water samples in Awka urban, Anambra State was conducted to determine their quality and suitability for domestic uses. A total of thirty shallow and deep well water samples were collected during the rainy and dry seasons. Methods: Physicochemical analysis was carried out using standard analytical methods. The total bacterial count was determined by dilution method. Results: Some of the physicochemical parameters (PH, dissolved oxygen, nitrate, cadmium, lead and arsenic) exceeded the World Health Organization maximum containment levels indicating that the samples were unfit for domestic uses. The bacterial counts ranged from 2.66 to 3.26 logcfu/ml during the rainy season and 2.54 to 3.20 logcfu/ml during the dry season. The total coliform counts also exceeded the W.H.O levels. Citrobacter freundii, Shigella flexneri, Serratia marcescens, Proteus vulgaris, Vibrio cholerae, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli and Bacillus subtilis were isolated during both seasons and identified using standard analytical procedures. The bacterium that had the highest frequency of occurrence during the rainy season was Citrobacter freundii (16.55%) while Salmonella typhi had the highest frequency of occurrence during the dry season (17.69%) respectively. Proteus vulgaris had the least frequency of occurrence 5.41% and 4.62% during the rainy and dry seasons respectively. The results were analyzed statistically using two-way analysis of variance. Higher bacterial counts were recorded in rainy season and shallow wells than dry season and deeper wells. Conclusion: The presence of these bacteria above admissible limits showed that the water lacked proper water management services and may be harmful to humans. There is therefore a need to monitor the well water quality by employing better sanitary practices and subjecting the waters through various forms of water treatments before use to help prevent disease outbreak.

acids and sulfonyl chloride. The characterization of the compounds was done using FTIR, 1H-NMR, 13C-NMR, and elemental analysis. Their antioxidant, antimalarial, antimicrobial activities and molecular docking were evaluated. Compounds 5h (MIC, 0.3, 0.5, 0.3 and 0.4 mg/mL) was the most potent antibacterial agent against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella typhi, compounds 5a (MIC, 0.7 mg/mL) and 5g (MIC, 0.7m g/mL) were most potent against Pseudomonas aeruginosa while compound 5e (MIC, 0.5 and 0.8 mg/mL) had the best in vitro antifungal activity against Aspergillus niger and Candida albicans. Compound 5d (1C50 = 1.128 μg/mL) displayed an antioxidant activity comparable with ascorbic acid (1C50 = 1.000 μg/mL) and compound 5e (4, 14, 4%) effected the most signifi cant reduction in the percentage malaria parasitaemia comparable with artemeter lumefantrin (5, 4, 4%) after 5 days of treatment of the mice. From the molecular docking results, compound 5c had a strong molecular interaction with the appropriate drug target, Escherichia coli DNA gyrase complexed with 1-ethyl-3-[8-methyl-5-(2-methyl-pyridin-4-yl) isoquinolin-3-yl]urea (PDB code: 5MMN). The title compounds displayed signifi cant antimicrobial, antioxidant and antimalarial activities.