In the presented work, a method was tested for determining the number of spermatozoa with intact acrosome in male producers using Diff-Quick dye. As a research method, we used a similar method for determining the integrity of the acrosome in breeding bulls developed in the FGBU «VGNKI» [5]. 25 samples of frozen sperm of canine producers were examined for the integrity of the sperm acrosome. Samples were provided by the clinical diagnostic center. The monitoring of the state of acrosomes in the sperm production of canine producers after staining with a specialized set of Diff-Quick dyes was carried out.
The research is focused on the standardization of nutrient media while using the microbiological method of veterinary and sanitary control of the quality of dog sperm for the presence of pathogenic microorganisms, namely, coliform bacteria. 25 samples of frozen sperm of sire males were examined for a microbiological indicator (determination of bacteria coliform group). The samples were provided by the clinical diagnostic center FGBU «VGNKI». During the research a comparative test of Bulir's nutrient medium was carried out. Also were carried out tests for other nutrient media recommended for isolating coliform bacteria.
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