Experimental data concerning complex study of antioxidant activity of Y. pestis with different plasmid spectrum (wild-type Yersinia pestis subsp. pestis, Yersinia pestis subsp. altaica and their isogenic variants) are represented in the article. Superoxide dismutase activity of the tested Y.pestis strains was from 6.0 to 9.0×109 microbe cells. Signifi-cant differences between the Y.pestis strains with different plasmid composition were not detected by this parameter. Our results, consistent with the data of other authors, tell that superoxide dismutase activity is a thermo-inducible feature and does nоt depend on a plasmid spectrum. High peroxide destroying activity was also detected in all tested Y.pestis strains. The differences between the strains regarding their common peroxide destroying activity were found. This parameter of the plague microbe strains lacking pYP plasmid was at least 3times lower than common peroxide destroying activity in strains with this plasmid in the genome. In our opinion these revealed differences were caused by characteristics of plasmid spectrum. The common peroxide destroying activity’s degree of Y.pestis strains can be associated with the presence of pYP plasmid in the genome. The isogenic variants of Y.pestis strains lacking one of the plasmids had smaller pathogenic activity. This fact points to the need for further study of these strains.
We studied the effect of an experimental synthetic organoselenium compound 2,6-dipyridinium-9-selenabicyclo[3.3.1]nonane dibromide (974zh) on the cell composition of the red bone marrow and peripheral blood in white mice. The study drug co-administered with Yersinia pestis EV vaccine strain (10 3 CFU) potentiated maturation and migration of mature neutrophils from the bone marrow into the circulation. Reducing the dose of the live vaccine and the anti-inflammatory properties of the study drug made it possible to reduce the allergic reaction during the vaccination process.
Получены экспериментальные препараты липополисахарида (ЛПС) Francisella tularensis четырех подвидов: tularensis, holarctica, mediasiatica, novicida методом твин-экстракции. Дана физико-химическая характеристика полученных препаратов. Изучено влияние ЛПС возбудителя туляремии разных подвидов на фагоцитарное звено иммунитета экспериментальных животных в условиях in vitro. Показано, что биомолекулы ЛПС проявляют слабое стимулирующее воздействие на кислород-и нитроксидзависимые бактерицидные системы и не обладают цитопатогенетическим свойством для активации апоптоза фагоцитов. Полученные результаты позволяют предположить, что такая инертность ЛПС возбудителя туляремии обусловлена особенностями его строения.
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