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Abstract:New simple, rapid, and accurate spectrophotometric techniques for sulphanilamides assay in combination drugs have been elaborated. The method is based on the formation of coloured products of sulphanilamides diazonium salt azoсoupling with heterocyclic azo reagent 4-(2-thiazolylazo)-resorcinol (TAR) in alkaline medium (pH 9.5) with the following measurement of their absorbance at λ max = 615 nm. Formed products are stable for 1 hour at room temperature. Effective molar absorptivities at absorbance maximum 615 nm for disazo dyes were ~ 10 4 M -1 cm -1 . Linear ranges for sulphanilamides determination were 0.5−30.0 µg mL -1 . The developed method was used for the suphanilamides determination in tablets, powder, solutions, suspensions and ointments.

A rapid, simple and sensitive spectrophotometric method for the determination of amoxicillin (AM) is described. The method is based on the previous sulphanilamide (SA) and sulphathiazole (STZ) diazotization in the medium of 0.6-0.7 M hydrochloric acid and their subsequent interaction with amoxicillin at pH = 10.5 with formation of yellow-colored azo compouds. Effective molar absorptivities at the absorbance maxima at 445 nm (SA) and 448 nm (STZ) for azo compounds were (1.74 ± 0.06) • 10 4 L × mol-1 × cm-1 and (1.97 ± 0.05) • 10 4 L × mol-1 × cm-1 , respectively. Stoichiometric ratios of the components of azo compounds were determined using continuous variations method. Based on the optimum reaction conditions, new methods were developed. These methods allow to determine the amoxicillin in concentration range 1.3-32.9 mg × mL-1 with sulphanilamide and 0.7-27.4 mg × mL-1 with sulphathiazole. The methods were successfully validated for amoxicillin determination in tablets "Amoxil".

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