Development and Validation of the Simple and Sensitive Spectrophotometric Method of Amoxicillin Determination in Tablets using Sulphanilamides

Abstract

A rapid, simple and sensitive spectrophotometric method for the determination of amoxicillin (AM) is described. The method is based on the previous sulphanilamide (SA) and sulphathiazole (STZ) diazotization in the medium of 0.6-0.7 M hydrochloric acid and their subsequent interaction with amoxicillin at pH = 10.5 with formation of yellow-colored azo compouds. Effective molar absorptivities at the absorbance maxima at 445 nm (SA) and 448 nm (STZ) for azo compounds were (1.74 ± 0.06) • 10 4 L × mol-1 × cm-1 and (1.97 ± 0.05) • 10 4 L × mol-1 × cm-1 , respectively. Stoichiometric ratios of the components of azo compounds were determined using continuous variations method. Based on the optimum reaction conditions, new methods were developed. These methods allow to determine the amoxicillin in concentration range 1.3-32.9 mg × mL-1 with sulphanilamide and 0.7-27.4 mg × mL-1 with sulphathiazole. The methods were successfully validated for amoxicillin determination in tablets "Amoxil".

A New Approach for the Voltammetric Determination of Amoxicillin in the Dosage Form Using Azo Coupling Reaction with Sulphanilamide

“…It should be noted that voltammetric studies made it possible to clarify the probable formula of the formed azo compound [32], which we have proposed on the basis of spectrophotometric studies.…”

“…According to the literature review, an azo coupling reaction between sulphanilamides diazonium salt and phenolic compounds occurs in an alkaline medium at pH 9-11 [31,32,35,36]. To develop a voltammetric technique, the optimal value of the acidity of the medium to obtain the maximum analytical signal was investigated.…”

“…Therefore, for further voltammetric studies, we have chosen pH 9.0. For the spectrophotometric determination of AM in the form of this azo compound, the optimal pH value is 10.5 [32].…”

“…SA as an analytical reagent has not been used before in the chemical analysis [31]. We were first to apply sulphanilamide for the spectrophotometric determination of AM [32].…”

Development and validation of spectrophotometric method and paper-based microfluidic devices for the quantitative determination of Amoxicillin in pure form and pharmaceutical formulations

“…Amoxicillin is used to treat various types of bacterial infections, for example pneumonia, pneumonia, and urinary tract infection, since it has the capability to inhibit the synthesis of bacterial cell walls [ 13 ]. Spectrophotometric [ [14] , [15] , [16] , [17] ], chromatographic [ [18] , [19] , [20] ] and electrochemical [ 21 , 22 ] techniques are among several methods reported for determining this drug in pharmaceutical preparations. Whilst current techniques are highly accurate and sensitive in general they fail to achieve the criteria of being low cost, rapid, or easy to operate that are required for high throughput screening [ 23 ].…”