The research was carried out in 2019–2020 in the Department of Biotechnology of RUE “Institute of Fruit-growing” in the laboratory conditions. The purpose of the study was to evaluate the efficiency of micro-grafting of blackberry varieties in ex vitro conditions. The carried out studies on the grafting of rooted ex vitro microshoots can be used to increase the commercial products output of blackberry varieties. During micro-grafting of blackberry varieties in ex vitro conditions, a 100 % output of rooted grafts was derived both on agroperlite substrate and on Sphagnum L. moss with a surface layer of peat “Dvinaˮ. With the complex of morphometric indicators, it is more expedient to use a substrate consisting of Sphagnum L. moss with a surface layer of peat “Dvinaˮ. An authentic influence was established with a high level of significance (p < 0.001) of both varietal characteristics and substrate and two factors together on the length of shoots, the number of internodes and the length of roots of blackberry varieties. The number of roots depended only on the type of substrate (p < 0.001).
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