miRNAs are endogenous approx 22 nucleotide RNA which mediates transcriptional or Post-transcriptional gene regulation and play a critical role in diverse aspects of plant development. miRNA identification in wet lab have various constraints, it is time consuming and expensive. It also faces the limitation of identifying miRNAs expressed at specific time and/or at special conditions. Due to the nature of strong conservation of miRNA in plant species, the use of comparative genomics approach for expressed sequence tags (ESTs), Genome Survey Sequence (GSS) and structural feature criteria filter has paved the way toward the identification of conserved miRNAs from the plant species whose genomes are not yet available in public domain. To identify the novel miRNA from Jatropha curcas, a total of 46862 EST sequences and 1569 GSS were searched for homology to previously known viridiplantae 2502 mature miRNA. After predicting the RNA secondary structure, 24 new potential miRNA were identified in J. curcas. Using the newly identified miRNA sequences, a total of 78 potential target genes were identified for 3 miRNA families. Most of the miRNA targeted genes were predicted to encode transcription factors that regulate cell growth and development, signaling, and metabolism. These findings considerably broaden the scope of understanding the functions of miRNA in J. curcas.
Cyanobacteria from habitats within Gujarat have been poorly studied with regard to their diversity. In the present investigation eight morphologically distinct cyanobacterial isolates were obtained and characterized from the fresh water and marine habitats. Identification was performed based on morphological features and on 16S rDNA sequences analysis. A phylogenetic tree based on 16S rDNA sequence of cyanobacterial isolates was prepared. Phylogenetic analysis clustered the eight morphologically distinct isolates into two distinct groups thus highlighting the importance of both morphological and genetic methods in studying cyanobacterial diversity.
miRNAs are 20-25 nucleotide in length, endogenous in origin, and noncoding RNA molecules found in all the eukaryotic organism and takes part in the regulation of gene expression. Jatropha curcas L. oil has drawn attention as one of the most potential biofuels that can be a substitute of never-ending fuel crisis. Six miRNA candidates, each belonging to different miRNA families were predicted after screening of 41,790 whole genome shotgun assembly sequences by using the comparative genomics approach. The targets of identified miRNA were also predicted, the predicted target genes are belonging to diverse functions like in metabolism, transport, and genes involved in fatty acid metabolism. The predicted miRNAs show their targets which involve in the fatty acid metabolism, like miR414 targets the gene of acetyl-CoA carboxylase and O-acyltransferase WSD1, miR846 targets gene of FAD omega-3 fatty acid, FAD plastid fatty acid desaturase, miR5658 have the target gene long chain acyl-CoA synthetase 1, and miR407 have the target gene Trigalactosyldiacylglycerol 2, miR2938 GDSL esterase/lipase, glycolipid transfer protein 1.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.