miRNA Identification from Jatropha curcas L. whole genome shotgun assembly sequences

Abstract

miRNAs are 20-25 nucleotide in length, endogenous in origin, and noncoding RNA molecules found in all the eukaryotic organism and takes part in the regulation of gene expression. Jatropha curcas L. oil has drawn attention as one of the most potential biofuels that can be a substitute of never-ending fuel crisis. Six miRNA candidates, each belonging to different miRNA families were predicted after screening of 41,790 whole genome shotgun assembly sequences by using the comparative genomics approach. The targets of identified miRNA were also predicted, the predicted target genes are belonging to diverse functions like in metabolism, transport, and genes involved in fatty acid metabolism. The predicted miRNAs show their targets which involve in the fatty acid metabolism, like miR414 targets the gene of acetyl-CoA carboxylase and O-acyltransferase WSD1, miR846 targets gene of FAD omega-3 fatty acid, FAD plastid fatty acid desaturase, miR5658 have the target gene long chain acyl-CoA synthetase 1, and miR407 have the target gene Trigalactosyldiacylglycerol 2, miR2938 GDSL esterase/lipase, glycolipid transfer protein 1.