Madhuca longifolia is the botanical name of the Mahua tree which belongs to the family Sapotaceae. Medicinally each part of the plant has specific importance and hence is cultivated for its medicinal use. The English synonym for Mahua is The Honey Tree. It is also been worshipped by tribal and has relevance in their norms and traditions (Saif et al., 2020). Mahua is also termed as a tree of Butter-nut and has many other synonyms in various languages like Atavimaduka in Sanskrit;
Aim: The purpose of this paper was to develop a sensitive, reproducible Reverse Phase High Performance Liquid Chromatography (RP-HPLC) for extraction and quantitative estimation of Scutellarein, major flavone glycoside from leaves of Triumfetta rhomboidea. Materials and Methods: To optimize best solvent system and ideal extraction methodology, various leaves extract was prepared by using different solvent systems such as ethanol, ethanol: water (50:50) and water through different extraction methodologies includes, maceration, Soxhlet assisted extraction (SAE), Ultrasound Assisted Extraction (UAE) and Accelerated Solvent Extraction (ASE). Chromatographic separation of Scutellarein was performed on C 18 column. Results:The results showed that the highest concentration of Scutellarein was found to be 1.547 ng in ethanolic extract. This study states about, existence of flavone glucocidic content in leaves extract of Triumfetta rhomboidea which can be extracted using ethanol as best solvent system and ASE as optimized extraction methodology. Conclusion: Extracts from T. rhomboidea obtained by maceration, SAE, UAE and ASE methods were analysed by HPLC-ESI-MS/MS to quantify flavonoid scutellarein contents. Ethanol was found to be a promising solvent system and Accelerated Solvent Extraction methodology was found to be the promising methodology for the extraction of Scutellarein from leaves of Triumfetta rhomboidea.
Background: This study’s purpose was to develop a sensitive, repeatable reverse-phase high-performance liquid chromatography (RP-HPLC) method for extracting the main fl avonoid Myricetin from the leaves of Madhuca longifolia and quantifying it. Materials and Methods: Diff erent leaves extracts were prepared using various solvent systems, including ethanol, ethanol: water (50:50), and water, through various extraction methodologies, including hot and cold maceration and soxhlet-assisted extraction, in order to optimize the best solvent system and ideal extraction methodology (SAE). Myricetin was separated by chromatography using a C18 column. Results: According to the fi ndings, the hydroalcoholic extract had the greatest content of myricetin at 2.524 ng. This study reports the presence of fl avonoid content in M. longifolia leaves extract that can be extracted using, water:ethanol and ethanol. The best solvent system was water:ethanol, and the best extraction technique was cold maceration. Conclusion: M. longifolia extracts were produced by hot and cold maceration, SAE techniques, and their fl avonoid concentrations were measured using HPLC-ESI-MS/MS. Water: Cold maceration and ethanol were shown to be promising solvent systems. While trying to extract myricetin from M. longifolia leaves, the extraction approach was more eff ective than other traditional extraction methods.
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