Biochemical Screening, Antibacterial and GC-MS Analysis of Ethanolic Extract of <i>Hemidesmus indicus</i> (L) R. Br. root.

Deshmukh, Madhuri M.; Ambad, Chhaya S.; Kendre, Nutan; Kashid, Navnath G.

doi:10.5958/0975-4385.2019.00014.1

Cited by 8 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aqueous extract was obtained by evaporating in a rotary evaporator. The whole residue was kept in airtight container at 4°C 20 and used for biochemical estimation and biological activities. A sterile environment was maintained throughout the whole experiment to ensure accuracy and efficacy.…”

Section: Preparation Of Plant Extractmentioning

confidence: 99%

Characterization, Synthesis, and Biological Activities of Silver Nanoparticles Produced via Green Synthesis Method Using Thymus Vulgaris Aqueous Extract

Ejaz,

Afzal,

Mazhar

et al. 2024

IJN

View full text Add to dashboard Cite

Introduction: Silver nanoparticles (AgNPs) have been found to exhibit unique properties which show their potential to be used in various therapies. Green synthesis of AgNPs has been progressively gaining acceptance due to its cost-effectiveness and energyefficient nature. Objective: In the current study, aqueous extract of Thymus vulgaris (T. vulgaris) was used to synthesize the AgNPs using green synthesis techniques followed by checking the effectiveness and various biological activities of these AgNPs. Methods: At first, the plant samples were proceeded for extraction of aqueous extracts followed by chromatography studies to measure the phenolics and flavonoids. The synthesis and characterization of AgNPs were done using green synthesis techniques and were confirmed using Fourier transform infra-red (FT-IR) spectroscopy, UV-visible spectroscopy, scanning electron microscope (SEM), zeta potential, zeta sizer and X-Ray diffraction (XRD) analysis. After confirmation of synthesized AgNPs, various biological activities were checked. Results: The chromatography analysis detected nine compounds accounting for 100% of the total amount of plant constituents. The FT-IR, UV-vis spectra, SEM, zeta potential, zeta sizer and XRD analysis confirmed the synthesis of AgNPs and the variety of chemical components present on the surface of synthesized AgNPs in the plant extract. The antioxidant activity of AgNPs showed 92% inhibition at the concentration of at 1000 µg/mL. A greater inhibitory effect in anti-diabetic analysis was observed with synthesized AgNPs as compared to the standard AgNPs. The hemolytic activity was low, but despite low concentrations of hemolysis activity, AgNPs proved not to be toxic or biocompatible. The anti-inflammatory activity of AgNPs was observed by in-vitro and invivo approaches in range at various concentrations, while maximum inhibition occurs at 1000 µg (77.31%). Conclusion:Our data showed that the potential biological activities of the bioactive constituents of T. vulgaris can be enhanced through green synthesis of AgNPs from T. vulgaris aqueous extracts. In addition, the current study depicted that AgNPs have good potential to cure different ailments as biogenic nano-medicine.

show abstract

Section: Preparation Of Plant Extractmentioning

confidence: 99%

Characterization, Synthesis, and Biological Activities of Silver Nanoparticles Produced via Green Synthesis Method Using Thymus Vulgaris Aqueous Extract

Ejaz,

Afzal,

Mazhar

et al. 2024

IJN

View full text Add to dashboard Cite

show abstract

Optimization of ultrasound-assisted extraction of bioactive chemicals from Hemidesmus indicus (L.) R.Br. using response surface methodology and adaptive neuro-fuzzy inference system

Vellur

Pavadai

Pandian

et al. 2023

Food Sci Biotechnol

View full text Add to dashboard Cite

Identification of Bioactive Compounds Present in Kulthi (Macrotyloma uniflorum) Seed Extract by Gas Chromatography-Mass Spectrometry

Abdullah

A.H

Fatema

et al. 2022

RJPT

View full text Add to dashboard Cite

The objectives of this research were to determine the chemical composition of the extract of Macrotyloma uniflorum seeds by using Gas chromatography–Mass Spectrometry analysis (GC-MS). GC-MS is a matchless method to study and measure the quantity of organic volatile and semi-volatile compounds. Gas chromatography is employed to separates mixtures into individual components employing a temperature-controlled capillary column. Mass spectrometry is utilized to recognize a variety of components from their mass spectra. In the present study, volatile/semivolatile compounds present inM. uniflorum seed extract were analyzed. M. uniflorum seed extract isextracted by the soxhlet extraction method and then analyzed by GC-MS. A total of 28 compoundswere found and quantified in this study. The major bioactive compounds in M. uniflorum seed extract are: 1,7-Octadiyne, 1-Propanone, 1-phenyl-3-[2-( phenylmethoxy)phenyl] -(16.93%), Benzene, 1-methyl-4-(1,2,2-trimethylcyclopentyl)-, (R)-, Benzene, 1-(1,5-dimethyl-4-hexenyl)- 4-methyl- (13.88%) and à-Farnesene, 1,3,6,10-Dodecatetraene, 3,7,11-trimethyl- (Z,E)- (12.68%). The present investigation revealed that M. uniflorum was composed of variety of metabolites and therapeutic active substances as well as novel substances. These substances could be isolated and further empirically evaluated to confirm their biologic and medicinal activities as well as investigate their mechanism of action.

show abstract

Biochemical Screening, Antibacterial and GC-MS Analysis of Ethanolic Extract of Hemidesmus indicus (L) R. Br. root.

Cited by 8 publications

References 0 publications

Characterization, Synthesis, and Biological Activities of Silver Nanoparticles Produced via Green Synthesis Method Using Thymus Vulgaris Aqueous Extract

Characterization, Synthesis, and Biological Activities of Silver Nanoparticles Produced via Green Synthesis Method Using Thymus Vulgaris Aqueous Extract

Optimization of ultrasound-assisted extraction of bioactive chemicals from Hemidesmus indicus (L.) R.Br. using response surface methodology and adaptive neuro-fuzzy inference system

Identification of Bioactive Compounds Present in Kulthi (Macrotyloma uniflorum) Seed Extract by Gas Chromatography-Mass Spectrometry

Contact Info

Product

Resources

About