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Introduction Gastrointestinal parasitic infections are one of the global health concerns in developing countries like Bangladesh. Among them, Cryptosporidium spp. plays an essential role in causing diarrhea, malnutrition, and poor cognitive function, especially in children. This study was conducted to identify the frequency of Cryptosporidium cases and other parasitic agents. Methods A cross-sectional observational study was conducted among 219 hospitalized children with diarrhea. The conventional microscopic technique was applied for parasitic detection. Particular staining (modified Ziehl-Neelsen) procedure was performed to identify oocysts of Cryptosporidium spp . A polymerase chain reaction (PCR) was performed to determine the SSU rRNA and gp60 gene of Cryptosporidium. Results Cysts of Giardia duodenalis (2.3%), ova of Ascaris lumbricoides (1.4%,), Trichuris trichiura (0.5%), and both A. lumbricoides and T. trichiura (0.9%) were identified in samples through wet mount preparation. The distribution of Cryptosporidium spp. as detected by the staining method and nested PCR was 1.4% and 4.1%, respectively. Conclusion Factors independently associated with Cryptosporidium infection are unsafe water, lack of regular hand washing, and insufficiency of exclusive breastfeeding. This study reports, presumably for the first time, the detection of Cryptosporidium oocysts in Chattogram metropolitan city of Bangladesh.

Background: Paediatric diarrhoea, the burning issue of nowadays, drastically affects the child mortality and morbidity. Apart from virus and bacteria, parasites also play an important role in causing diarrhoea at that age group. Giardiasis, the most prevalent among the parasitic infections, though neglected yet carries significance because of its long term health issues. The aim of the study was to identify the intestinal parasites by wet mount preparation and further detection of the flagellated protozoan parasite, Giardia by special staining - Trichrome staining technique. Materials and methods: It was an observational cross sectional study which was carried between the periods of July 2019 to June 2020, where a total of 200 diarrhoeic patients up to 18 years were enrolled. Direct microscopic method was done where parasites were detected by wet mount preparation and further Trichrome stainning was performed for the detection of Giardia. Results: Out of 200 faecal samples, cysts of Giardia, ova of Ascaris Lumbricoides (AL) Trichuris Trichiura (TT) and both AL and TT were identified in 2.5%, 1.5%, 0.5% and 1.0% samples respectively by wet mount preparation. By Trichrome staining method we detected 3.5% of Giardia cysts. Conclusion: Staining technique could be applied in addition to wet mount preparation of stool samples. So that we could recover the undetected Giardia cyst in the aforesaid samples. JCMCTA 2021 ; 32 (2) : 111-114

Background: Gastrointestinal parasitic infections are one of the global health concerns in developing countries like Bangladesh. Among them, Cryptosporidium spp. plays an essential role in causing diarrhea, malnutrition, and poor cognitive function, especially in children. The study was conducted to identify the frequency of cryptosporidium cases and other parasitic agents. Methods: A Cross-sectional observational study was conducted among 219 hospitalized children with diarrhea. The conventional microscopic technique was applied for parasitic detection. A particular staining procedure was performed to identify oocysts of Cryptosporidium spp. And PCR was conducted to determine the SSU rRNA and gp60 gene of Cryptosporidium. Results: Cyst of Giardia, ova of Ascaris lumbricoides (AL), Trichuris trichiura (TT), AL, and TT were identified in 2.3%, 1.4%, 0.5%, and 0.9% samples by wet mount preparation. The distribution of Cryptosporidium spp. was 1.4% and 4.1%, which was detected by the staining method and nested PCR. Factors independently associated with Cryptosporidium infection are unsafe water, lack of regular hand washing, and insufficiency of exclusive breastfeeding. Conclusions: This is the first report to detect the frequency of Cryptosporidium and other intestinal parasites and associated factors in Chattogram city of Bangladesh.