Some important genes are involved in controlling the reproductive trait of goats, one of which is the Melatonin Receptor 1A (MTNR1A) gene. The MTNR1A gene is known with seasonal reproductive activity and related to lambing frequency in the goat. The purpose of this research was to determine the genetic polymorphisms of the MTNR1A gene in Kacang, and Peranakan Ottawa (PE) goat population reared traditionally in South Sulawesi region of Indonesia. In total 253 heads of goat consist of 137 heads of Kacang and 116 Peranakan Ottawa from South, Sulawesi region was used as research samples for blood collection. The blood samples were collected from the jugular vein, which was then continued for DNA extracted by using a DNA extraction kit. The MTNR1A genotype was identified by PCR-RFLP technique using restriction enzyme RsaI. The result showed that there was genetic diversity in the MTNR1A gene in Kacang and PE population with the obtained of two alleles R and r. The common allele was R with frequency 0.93 in Kacang population while in PE population was 0.89. The r allele was 0.06 and 0.10 in Kacang and PE population, respectively. The most common genotype found in the population was RR (0.95), while Rr was only 0.05 and rr genotype did not found in this population. Observed heterozygosity value was 0.05. According to the Hardy-Weinberg test, this population was in equilibrium for MTNR1A gene. In conclusion, this finding indicated that there was genetic diversity exists in local goat population, and future research needed to find any association with this genetic variation with reproductive performance, the data obtained from this study could be used for the strategic program in goat breeding to increase reproductive performance of local goat especially fertility traits.
This study aims to determine the effect of the preparation method and the number of samples on the quality of purified DNA from beef sausage. The study was conducted using a Randomized Block Design. The main factor is the sample preparation method which consists of P1: fresh sample; P2: oven-dried sample; and P3: Freeze-drying dry samples. Each treatment consisted of 4 groups, namely K1: sample weight 25 mg; K2: sample weight 50 mg; K3: sample weight 75 mg; and K4: sample weight 100 mg. Each treatment group was repeated 2 times so that the total sample was 24. The results showed that DNA purification using different preparation methods and sample weights was successfully carried out, as seen from the presence of DNA bands visualized on agarose gel with EtBr staining. The average DNA quality of the P1, P2, and P3 preparation methods were 1.60±0.90 ng/μl, 2.63±0.99 ng/μl, and 2.94 ±0.89, ng/μl respectively, with DNA purity 1.023 ±0.165, 0.937±0.148, and 1.014±0.163. The average DNA quality at K1, K2, K3, and K4 obtained DNA concentrations of 3.03±1.64 ng/μl, 3.15±0.74 ng/μl, 2.28± 1.66 ng/μl, and 2.54±1.50 ng/μl with a purity of 1.059±0.142, 0.981±0.130, 0.908±0.061, and 1.061± 0.215. The average total concentration of purified DNA from beef sausage was 2.75±1.28 ng/μl with a purity of 0.991±0.149. The results of variance showed that the treatment did not affect the concentration and purity of purified DNA from beef sausage. This study concludes that DNA purification from beef sausage can be carried out, but the preparation method and number of samples do not affect the quality and quantity of DNA.
Tujuan penelitian ini untuk mengetahui pengaruh ekstrak kulit buah naga terhadap kualitas semen ayam kampung. Penelitian ini menggunakan Rancangan Acak Lengkap yang terdiri dari 4 perlakuan dan 3 ulangan. 12 ekor ayam Kampung jantan dibagi secara acak dalam 4 kelompok perlakuan. Sebelum perlakuan, seluruh ayam diadaptasikan selama 10 hari dan hanya diberi pakan komersil dan air minum. Perlakuan diberikan secara oral selama 8 minggu dan terdiri dari P0 = Kontrol, P1= Ekstrak kulit buah naga 1ml, P2= Ekstrak kulit buah naga 3ml, dan P3= Ekstrak kulit buah naga 5ml kemudian dilakukan penampungan semen dengan metode pengurutan. Sperma yang telah ditampung kemudian diuji kualitasnya di Laboratorium. Parameter yang diukur yaitu kualitas semen secara makroskopis meliputi warna, bau, konsistensi, volume, dan pH semen. Hasil penelitian menunjukkan bahwa pemberian ekstrak kulit buah naga tidak memberikan pengaruh yang nyata terhadap warna, bau, konsistensi, dan pH semen tetapi memberikan pengaruh pada volume semen yaitu volume semen ayam yang diberi perlakuan lebih tinggi dibandingkan dengan ayam yang tidak diberi perlakuan. Secara keseluruhan semen yang diperoleh memiliki kualitas yang baik dan normal baik yang diberi perlakuan maupun yang tidak diberi perlakuan.
Tujuan penelitian ini untuk mengetahui pengaruh suplementasi ekstrak daun kelor (Moringa oleifera) terhadap kualitas makroskopis spermatozoa ayam kampung. Penelitian ini menggunakan 15 ekor ayam kampung jantan berumur + 1 tahun dibagi secara acak dalam 3 kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Sebelum diberi perlakuan, seluruh ayam diadaptasikan selama 1 minggu dan hanya diberi pakan komersil dan air minum. Perlakuan diberikan secara oral pada pagi hari selama 45 hari dan terdiri dari P1 : Ekstrak Daun Kelor 3 ml, P2 : Ekstrak Daun Kelor 6 ml, dan P3 : Ekstrak Daun Kelor 9 ml. Setelah dipelihara dan diberi perlakuan selama 45 hari, dilakukan penampungan sperma. Teknik penampungan sperma pada ayam dilakukan dengan menggunakan metode massage (metode pemijatan) pada bagian punggung ayam. Sperma yang telah ditampung kemudian diperiksa kualitasnya di Laboratorium. Parameter yang diukur yaitu kualitas sperma secara makroskopis meliputi volume, pH, warna, konsistensi, dan bau sperma. Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak daun kelor tidak berbeda nyata terhadap volume, pH, warna, konsistensi, dan bau sperma (P>0,05) tetapi seluruh kualitas sperma yang diperoleh setelah pemberian ekstrak daun kelor termasuk dalam kategori yang normal dan baik.
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