AbstrakBiosensor DNA elektrokimia bagi pengesanan jujukan DNA porsin berasaskan kompleks rutenium (II) sebagai label aktif redoks telah dibangunkan. Sistem biosensor difabrikasi berdasarkan elektrod bercetak skrin (SPE) terubahsuai dengan zarah nano emas (AuNPs) sebagai transduser yang dipegunkan bersama mikrosfera poli (n-butilakrilat-N-akriloksisuksnimida) dan jujukan DNA prob terikat melalui ikatan kovalen. Kompleks interkalator rutenium (II), [Ru(bpy) 2+ dan DNA prob, masing-masing adalah 50 µM dan 2 µM. Masa yang optimum bagi pemegunan DNA prob dan penghibridan DNA sasaran adalah 7 jam dan 60 minit. Keadaan optimum bagi pH, kekuatan ion, kepekatan larutan penimbal dan suhu, masing-masing adalah pH 7.0, 1.0 M NaCl, 0.05 M potassium fosfat pada suhu 25°C. Julat linear bagi kepekatan DNA sasaran yang berbeza adalah antara 1.0 x 10 -13 M to 1.0 x 10 -8 M. Kajian ini adalah yang pertama melaporkan tentang penggunaan [Ru(bpy) 2 PIP] 2+ bagi pengesanan DNA porsin Kata kunci: biosensor, DNA porsin, kompleks rutenium bipiridina Abstract Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles (AuNPs) as transducer immobilized with poly(n-butylacrylate-N-acryloxysuccinimide) microsphere and porcine DNA probe sequences was attached onto it via the covalent bond. The ruthenium (
An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy) 2 (PIP)] 2+ , (bpy = 2,2'bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy) 2 (PIP)] 2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy) 2 (PIP)] 2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10-5 uM to 1x10-13 uM.
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