Fabrication of Porcine DNA Biosensor Based on Ruthenium Bipyridine Complex

Halid, Nurul Izni Abdullah; Zakariah, Emma Izzati; Lee, Yook Heng; Karim, Nurul Huda Abd; Ahmad, Hasan; Hasbullah, Siti Aishah

doi:10.17576/mjas-2016-2005-06

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…An electrochemical DNA biosensor using gold-modified SPCE electrodes with an AuNP/DNA bioconjugate was developed to detect mtDNA S. scrofa with methylene blue as an indicator, and can detect 10% content in a mixture of processed meat [ 28 ]. The use of a ruthenium hexamine molecule as an indicator was also reported [ 29 ], along with that of a ruthenium (II) complex, [Ru(bpy) 2 PIP] 2+ , (bpy = 2, 2′-bipyridine, PIP = 2-phenylimidazo [4,5-f][1,10-phenanthroline]) as the label redox complex [ 30 ]. An electrochemiluminescence method was also developed for the detection of pork with a low limit of detection [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

The Optimization of a Label-Free Electrochemical DNA Biosensor for Detection of Sus scrofa mtDNA as Food Adulterations

et al. 2023

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Fast, sensitive, and easy-to-use methods for detecting DNA related to food adulteration, health, religious, and commercial purposes are evolving. In this research, a label-free electrochemical DNA biosensor method was developed for the detection of pork in processed meat samples. Gold electrodeposited screen-printed carbon electrodes (SPCEs) were used and characterized using SEM and cyclic voltammetry. A biotinylated probe DNA sequence of the Cyt b S. scrofa gene mtDNA used as a sensing element containing guanine substituted by inosine bases. The detection of probe-target DNA hybridization on the streptavidin-modified gold SPCE surface was carried out by the peak guanine oxidation of the target using differential pulse voltammetry (DPV). The optimum experimental conditions of data processing using the Box–Behnken design were obtained after 90 min of streptavidin incubation time, at the DNA probe concentration of 1.0 µg/mL, and after 5 min of probe-target DNA hybridization. The detection limit was 0.135 µg/mL, with a linearity range of 0.5–1.5 µg/mL. The resulting current response indicated that this detection method was selective against 5% pork DNA in a mixture of meat samples. This electrochemical biosensor method can be developed into a portable point-of-care detection method for the presence of pork or food adulterations.

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Section: Introductionmentioning

confidence: 99%

The Optimization of a Label-Free Electrochemical DNA Biosensor for Detection of Sus scrofa mtDNA as Food Adulterations

et al. 2023

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Current analytical methods for porcine identification in meat and meat products

et al. 2020

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Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

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Zakariah

Ruslin

et al. 2021

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Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because of its stable thermodynamic, kinetic and redox properties. Novel hexaferrocenium tri[hexa(isothiocyanato)iron(III)]trihydroxonium (HexaFc) complex was the product from the reaction of ferrocene, maleic acid and ammonium thiocyanate and was confirmed by elemental analysis CHNS, FTIR and single crystal X-ray crystallography. In this study, HexaFc was used for the first time as an electroactive indicator for porcine DNA biosensor. The UV–Vis DNA titrations with this compound showed hypochromism and redshift at 250 nm with increasing DNA concentrations. The binding constant (Kb) for HexaFc complex towards CT-DNA (calf-thymus DNA) was 3.1 × 104 M−1, indicated intercalator behaviour of the complex. To test the usefulness of this complex for DNA biosensor application, a porcine DNA biosensor was constructed. The recognition probes were covalently immobilised onto silica nanospheres (SiNSs) via glutaraldehyde linker on a screen-printed electrode (SPE). After intercalation with the HexaFc complex, the response of the biosensor to the complementary porcine DNA was measured using differential pulse voltammetry. The DNA biosensor demonstrated a linear response range to the complementary porcine DNA from 1 × 10−6 to 1 × 10−3 µM (R2 = 0.9642) with a limit detection of 4.83 × 10−8 µM and the response was stable up to 23 days of storage at 4 °C with 86% of its initial response. The results indicated that HexaFc complex is a feasible indicator for the DNA hybridisation without the use of a chemical label for the detection of porcine DNA.

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Fabrication of Porcine DNA Biosensor Based on Ruthenium Bipyridine Complex

Cited by 4 publications

References 15 publications

The Optimization of a Label-Free Electrochemical DNA Biosensor for Detection of Sus scrofa mtDNA as Food Adulterations

The Optimization of a Label-Free Electrochemical DNA Biosensor for Detection of Sus scrofa mtDNA as Food Adulterations

Current analytical methods for porcine identification in meat and meat products

Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

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