ABSTRACT. We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Development Center, and a sample of S. pimpinellifolium were genotyped with RAPD and SRAP markers. Eleven SRAP primer combinations were used and 66 bands were scored. The number of bands scored per primer combination ranged from three to 12, with a mean of six alleles per primer combination. All fragments scored for each primer combination were polymorphic. The percentage of polymorphic products ranged from 25 to 80%. The 15 tomato genotypes were screened for RAPD markers using 50 primers in a PCRbased DNA amplification procedure; 46 primers produced clear and good amplification. Ten of these 46 primers amplified monomorphic 2264 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 2263-2274 (2010) N. Comlekcioglu et al. fragments in the tomato genotypes. A dendrogram was constructed by combining data from the RAPD and SRAP analyses. Similarity ratios of genotypes ranged from 0.87 to 0.99. The dendrogram was divided into two branches; the first main branch included only genotype CL 5915, and the second main branch included all the other genotypes.

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In vitro androgenesis methods are significant in rapid production of completely homozygous lines. Factors such as nutrient medium composition, types and concentrations of carbohydrate sources are effective on androgenetic success. Different sugar types were tested at different doses in anther culture of pepper to elucidate their influence on the yield of androgenesis. In this study, maltose was used as a source of sugar at 1.5, 3 and 6% doses. 3% sucrose was used as the control treatment. Murashige & Skoog (1962-MS) medium and 0.25% activated charcoal, 15 mg L-1 silver nitrate (AgNO3), 4 mg L-1 naphthalene acetic acid (NAA), and 2 different amounts of 6-benzyl amino purine (0.5, 1 mg L-1 BAP) with three breeding lines of pepper were used to know the genotypic effects on haploid embryo formation. When comparing 1.5, 3 and 6% maltose and 3% sucrose with each other in the medium, embryo response increased with maltose concentration. Maltose, which was generally compare to sucrose as carbohydrate source for anther culture, the embryo response had significantly affected at maltose concentrations. The combination with the best effect among the trial parameters was 30 g L-1 maltose and 0.5 mg L-1 BAP' for obtaining embryos from anther culture. Also, it was found that genotype is one of the main factors affecting success in pepper anther and microspore culture.

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