The specific purpose of this activity is to increase the motivation of women in improving the quality of life and self-reliance in the family by optimizing the yard with organic vegetable farming in the lowlands based on Local Wisdom and Sustainable. The strategy used was the empowerment of target groups with the approach of Participatory Rural Appraisal, methods of education, training, demonstration plots, assistance and equipped with learning by doing techniques. The materials presented were: (1) garden intensification technology with verticultur method, (2) organic vegetable cultivation technology based on liquid organic fertilizer, botanical pesticide and PGPR, (3) composting technology with enrichment of Trichoderma harzianum (Tricho-compos) activator of local microorganism, (4) technology of pest and disease control on environmentally friendly vegetable crops using botanical insecticides and microbial antagonists; (5) water use efficiency technology with drip irrigation; (6) good sorting, grading and packing technology, 7) empowerment technology to form groups and institutional strengthening and group management. The results of this activity are: (1) the target group / members The women farmer group has a very high curiosity about new information that can improve their standard of living,(2) technology transfer activities on the use of garden with organic vegetable cultivation received positive response from the members of the Group, (3) This devotional activity can encourage the pattern of mind and pattern of member action in organic vegetable cultivation by developing rural resources and sustainable local wisdom, (4) Verikultur cultivation technique with vertical paralon type on a land area of 10 m2 is economically profitable
Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SD-CASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively. Key words:Bacillus subtilis, siderophore, cathecolate, hydroxamate, growth of eggplants, inhibition to pathogens ABSTRAK Aktivitas siderofor Bacillus subtilis sebagai pemacu pertumbuhan dan pengendali patogen tanaman terung. Penelitian ini bertujuan untuk mengidentifikasi siderofor dari B. subtilis, menilai aktivitasnya sebagai pemacu pertumbuhan, dan pengendali patogen tanaman terung. Lima isolat B. subtilis yaitu B46, B209, B211, B298 dan B 315 ditumbuhkan pada medium SD-CASA. Isolat yang dapat memproduksi siderofor terbaik ini selanjutnya diuji kemampuannya sebagai pemacu pertumbuhan tanaman terung pada dua jenis tanah dengan kandungan Fe berbeda. Uji penghambatan dilakukan terhadap dua macam patogen, yaitu Colletotrichum sp. dan Ralstonia solanacearum. Percobaan di rumah kaca dirancang menggunakan Rancangan Acak Kelompok Faktorial dengan 2 faktor. Faktor pertama adalah B. subtilis (dengan B. subtilis B298 dan tanpa B. subtilis B298), faktor ke dua adalah jenis tanah (Ultisol dan Andisol). Variabel yang diamati antara lain serapan Fe oleh tanaman dan parameter pertumbuhan tanaman terung meliputi tinggi tanaman, jumlah daun, panjang akar, volume akar, bobot segar dan kering tanaman serta bobot segar dan kering akar, persentase penghambatan terhadap jamur dan bakteri pat...
Abstract. Mugiastuti E, Suprayogi, Prihatiningsih N, Soesanto L. 2020. Short Communication: Isolation And Characterization Of The Endophytic Bacteria, And Their Potential As Maize Diseases Control. Biodiversitas 21: 1809-1815. Sheath blight and bacterial wilt are diseases that can reduce maize production. Biological control with the endophytic bacteria offers environmentally friendly control for these pathogens. The study aimed to isolate and characterize the endophytic bacteria morphologically and biochemically and to study their potential to control maize diseases, especially sheat blight and bacterial wilt causing pathogens. The study was conducted at the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University, from April to August 2019. The study consisted of four stages: isolation and characterization of endophytic bacteria, the antagonism test of the endophytic bacterial to R solani, the antagonism test of the endophytic bacteria to Pantoea sp., and the mechanical test of the endophytic bacteria as biological control agents and plant growth-promoting bacteria. Based on the research, four endophytic bacteria isolates have been successfully isolated, and characterized successfully and found have the potential to be developed as biopesticides to control maize disease, especially R. solani and Pantoea sp. Bacillus sp, endophytic from the root (BK.A1; BK.A3; PP.A5) and Bacillus sp. endophytic from the stem (PPD.B2) can suppress the growth of R.solani by more than 50%, have a strong antagonistic index against Pantoea sp (> 4), and can produce protease and lipase enzyme, and phosphate solubilization.
Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B. subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.Key words: antibiosis, Bacillus subtilis B315, bacterial wilt disease control, potato plant growth promote ABSTRAKMekanisme antibiosis Bacillus subtilis b315 untuk pengendalian penyakit layu bakteri kentang. Bacillus subtilis B315 yang diisolasi dari rizosfer kentang mempunyai mekanisme antibiosis terhadap Ralstonia solanacearum secara in vitro. Mekanisme antibiosis secara in vitro ini sebagai potensi B. subtilis B315 untuk mengendalikan penyakit layu bakteri di lahan kentang. Tujuan penelitian adalah untuk mempelajari mekanisme antibiosis B. subtilis B315 untuk pengendalian penyakit layu bakteri kentang, dan mempelajari potensi B. subtilis B315 sebagai pemicu pertumbuhan tanaman kentang, serta mengevaluasi mekanisme B. subtilis B315 sebagai agensia pengendali hayati. Metode yang digunakan adalah percobaan di rumah kaca dengan Rancangan Acak Lengkap dengan 5 perlakuan dan 6 ulangan. Perlakuannya adalah kontrol (tanpa B. subtilis B315), B. subtilis B315 tipe alami, mutan antibiosis M16, mutan antibiosis M4, mutan antibiosis M14. Variabel yang diamati adalah masa inkubasi, indeks penyakit, laju infeksi, efektivitas pengendalian, komponen pertumbuhan tanaman (jumlah tunas, tinggi tanaman, luas daun, bobot brangkasan segar dan kering). Hasil penelitian menunjukkan bahwa B. subtilis B315 sebagai agensia pengendali hayati penyakit layu bakteri kentang dapat menunda masa inkubasi, menekan indeks penyakit layu bakteri dengan efektivitas 64,9%, menekan laju infeksi dan mampu memicu peningkatan pertumbuhan tanaman yang dilihat dari luas daun. B. subtilis B315 sebagai agensia pengendali hayati mempunyai mekanisme antibiosis dan m...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.