Vol. 30 acid (XIV). The analytical sample was purified by four recrystallizations from acetone: m.p. 192-194°14; t.l.c., ethyl acetate-acetone-methanol (3:3:1). Mixture melting point and infrared (KBr) were coincident with an authentic specimen.
Para-nitrobenzoic acid lnay serve as an oxidizable substrate in addition to other metabolic functions in microorganisms. A strain of Psezrdamanas$zraresce?zs is capable of using this compound as a sole source of organic carbon and nitrogen for aerobic growth. The enzyme responsible for the oxidation of p-nitrobenzoic acid is adaptive in nature. Experimental results with living, irradiated, and dried-cell preparations indicate that p-aminobenzoic acid, p-hydrosybenzoic acid, and protocatechuic acid are intermediates in the oxidative scheme and the sequence of appearance is in this order. Several other compounds were studied but could not be implicated in the scheme. The nitro group apparently is reduced in vivo to the corresponding amino form. The amino group is then removed as ammonia. By analogy, intermediates in the metabolic scheme of organo nitro compounds might well elucidate the pathway of nitrate reduction.
Materials and MethodsThe organism used throughout this study was a strain of P. jluorescens capable of utilizing 9-nitrobenzoic acid as a sole source of organic carbon and nitrogen for aerobic growth. Simultaneous adaptation (7) was employed Manuscript
A small series of pyrazoles and isoxazoles derived from thiochroman-4-one has been synthesized and characterized. The compounds were examined for their in vitro inhibitory activity against Bacillus subtilis and Pseudomonas fluorescens. Among the tested compounds the pyrazole derivative from thiochroman-4-one was found to be the most effective inhibitor of growth of B. subtilis. Extensive H NMR analysis was recorded for all compounds.
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