Endophytic fungi were isolated from black spruce (Picea mariana (Mill.) BSP) dormant buds and needles of four age-classes. Only one isolate was obtained from 400 buds. A total of 914 isolates were obtained from the needles. With increasing needle age the rate of colonization increased between current-year and 3-year-old needles from 4 to 90%, respectively. The needle segment attached to the twig was colonized more often (p < 0.05) than other segments overall and for 6 of the 11 taxa isolated. The first endophyte from current-year needles was isolated on July 14, 1988, but endophytes were obtained from needles of the other age-classes (1, 2, and 3 years) on all sampling dates between June 10 and September 16, 1988. Key words: endophyte, Picea mariana, dormand buds, needles.
1989. An investigation of needle endophyte colonization patterns with respect to height and compass direction in a single crown of balsam fir (Abies balsamea). Can. J. Bot. 67: 723-725. All branches of a balsam fir (Abies balsamea (L.) Mill.) were removed and twenty 3-year-old needles from each branch were surface sterilized and placed on 2 % malt extract media. There was a trend for obtaining more isolates of endophytic fungi at the bottom of the crown than at the top (r2 = 0.87). Phyllosticta sp. and Leptostroma sp. were isolated most often and were the most prominent by virtue of having more isolates for each branch sampled. No significant difference was found between compass directions for the four most frequently isolated species. JOHNSON, J. A., et WHITNEY, N. J. 1989. An investigation of needle endophyte colonization patterns with respect to height and compass direction in a single crown of balsam fir (Abies balsamea). Can. J. Bot. 67 : 723-725. Toutes les branches d'un sapin baumier (Abies balsamea (L.) Mill.) ont Ct C dCtachCes et 20 aiguilles agtes de 3 ans, prClevCes sur chaque branche, ont Ctt superficiellement stCrilisCes avant d'&tre placCes sur extrait de malt A 2% gClosC. Une tendance se dessine montrant un plus grand nombre de champignons endophytiques A la base de la couronne qu'au sommet (r2 = 0.87). Phyllosticta sp. et LRptostroma sp. ont Ctt le plus frequemment isolCs et se sont avCrts les plus prokminents en montrant plus d'isolats pour chaque branche Cchantillonnte. Aucune diffkrence significative n'est apparue entre les point cardinaux pour les quatre especes les plus frkquentes.[Traduit par la revue]
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