mixture, the color reactions would indicate malonic acid tb the exclusion of the other two acids.In practice the technique should be used as follows:Develop a chromatogram of the unknoffn material and spray with bromophenol blue. Calculate the RF values for each spot.From these RF values, select acids for standards of comparison which have corresponding RF values. Prepare new chromatograms in quadruplicate, so that each standard acid is run adjacent to an unknown mixture. After drying the paper sheets, spray each chromatogram with the appropriate color-producing reagent and treat and observe as described under "Materials and Reagents."
ACKNOWLEDGMENTThe authors sincerely thank Eric Baer, University of Toronto, for furnishing the sample of glyceric acid, J. W. E. Chattfield, University of Chicago, for furnishing the samples of a, 0-, and a,-pdihydroxybutyric acids, and various members of the laboratory staff for supplying other acids.
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LlTERATURE CITED(1) "Allen's Commercial
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