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In this study, a solvent extraction technique was investigated for the recovery of hydrocarbons from a concentrated slurry of Botryococcus braunii via thermal pretreatment. Efficient hydrocarbon recovery was achieved by preheating the slurry to temperatures below 100℃ to achieve the separation of solids (solid-liquid separation), followed by the solvent extraction of hydrocarbons. This procedure did not require a highly energy consuming drying process. Parameters affecting the energy profit ratio and economy of hydrocarbon recovery, such as heating temperature, water content after solid-liquid separation, extraction time, and solvent-to-algae ratio, were examined with respect to hydrocarbon recovery efficiencies; it was found that heating the mixture to below 100 ℃ was sufficient for good hydrocarbon recoveries. It was also shown that solvent extraction carried out at room temperature required 2 h to reach saturation. At least 90% of the hydrocarbons could be recovered, even with low solvent-toalgae ratios, by adjusting the algae water content to around 85 wt%.

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- - 研究論文 白色腐朽菌由来リグニンペルオキシダーゼ遺伝子 およびマンガンペルオキシダーゼ遺伝子の検出木材保存(Wood Preservation)37(3) ，111-121(2011) Detection of the genes encoding lignin and manganese peroxidases from white rot fungi White rot fungi produce lignolytic enzymes to depolymerize lignin when they degrade wood. Among them, heme peroxidases such as manganese peroxidase(MnP)and lignin peroxidase(LiP)have been considered to play an important role in lignin degradation. In the present study, the technique that permits a specific amplification of the genes encoding MnP and LiP by polymerase-chain reaction(PCR)was developed to detect the white rot fungi having these genes. As a result, the genes encoding MnP and LiP from white rot fungi including Trametes versicolor, Pycnoporus cinnabarinus, Pycnoporus coccineus, and Irpex lacteus were detected using newly-designed primers(MnP_F1 and MnP_R1) . Moreover, various peroxidase genes were also detected when DNAs prepared from decayed woods were used as a template. The genes encoding MnP and LiP were remarkably separated when PCR products were applied for denaturing gradient gel electrophoresis analysis. The results clearly indicate that the technique is useful when naturally-occurring decayed wood containing various gene resources encoding MnP and LiP was used as a subject of research.Key words：white rot fungi, lignin peroxidase, manganese peroxidase, denaturing gradient gel electrophoresis ＊１ 東京農工大学 農学部 環境資源科学科

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