The present study evaluates the protective role of Quercetin (Quer), against immobilization stress- induced anxiety, depression and cognition alteration in mice using behavioral and biochemical parameters. 24 adult Albino mice were distributed into 2 groups vehicle (n=12; 1 ml/kg) and Quer injected (n=12; 20 mg/kg/ml). The animals received their respective treatment for 14 days. On day 15, after the drug administration, animals were sub-divided into 4 groups (n=6); (i) unstressed + vehicle; (ii) stressed + vehicle; (iii) unstressed + Quer; (iv) stressed + Quer. On day 16, 24 h after the immobilization stress behavioral activities (light-dark activity, elevated plus maze, Morris water maze, and forced swim test) monitored and then animals were decapitated 1 h after the drug administration. Brain samples were collected for biochemical (antioxidant enzymes, AChE, ACh, 5-HT and its metabolite) analysis. The present study indicates the Quer reversed the stress-induced anxiety and depression, in addition, memory performance was more enhanced in stressed group. Following the treatment of Quer, stress-induced elevation of lipid peroxidation and suppression of antioxidant enzymes were also reversed. Administration of Quer decreased AChE in unstressed, while levels of acetylcholine were increased in vehicle and Quer treated stressed animals. The metabolism of 5-HT was increased in Quer treated stressed than unstressed animals. In conclusion, the present finding showed that Quer could prevent the impairment of antioxidant enzymes and also regulate the serotonergic and cholinergic neurotransmission and produce antianxiety, antidepressant effect and enhance memory following 2 h immobilization stress in mice.

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In 30% of epileptic individuals, intractable epilepsy represents a problem for the management of seizures and severely affects the patient's quality of life due to pharmacoresistance with commonly used antiseizure drugs (ASDs). Surgery is not the best option for all resistant patients due to its post-surgical consequences. Therefore, several alternative or complementary therapies have scientifically proven significant therapeutic potential for the management of seizures in intractable epilepsy patients with seizure-free occurrences. Various non-pharmacological interventions include metabolic therapy, brain stimulation therapy, and complementary therapy. Metabolic therapy works out by altering the energy metabolites and include the ketogenic diets (KD) (that is restricted in carbohydrates and mimics the metabolic state of the body as produced during fasting and exerts its antiepileptic effect) and anaplerotic diet (which revives the level of TCA cycle intermediates and this is responsible for its effect). Neuromodulation therapy includes vagus nerve stimulation (VNS), responsive neurostimulation therapy (RNS) and transcranial magnetic stimulation therapy (TMS). Complementary therapies such as biofeedback and music therapy have demonstrated promising results in pharmacoresistant epilepsies. The current emphasis of the review article is to explore the different integrated mechanisms of various treatments for adequate seizure control, and their limitations, and supportive pieces of evidence that show the efficacy and tolerability of these non-pharmacological options.

Grewia asiatica L. fruit natively called phalsa is a popular berry of Pakistan and widely consumed in the form of fresh juices and carbonated drinks in the summer season. The berry is enriched with antioxidants such as phenols, flavonoids, anthocyanins, and vitamin C. Scientifically, it is the least explored berry in terms of neuromodulatory activities, and therefore, in the designed study, chronically fed rats with the different dilutions (5%−30%) of fruit juice were subjected to behavioral assessment for anxiety, depression, and cognition (spatial memory) followed by biochemical analysis of isolated brains. Results revealed a prominent impact of 20 and 30% dilutions of fruit exudate as treated animals showed anxiolytic behavior to central zone (P < 0.05) of open field test (OFT) and open arms of elevated plus maze (EPM) (P < 0.05) in anxiety models. Overall, immobility of rats treated with a higher concentration of exudate in forced swim test (FST) was reduced (P < 0.05) presenting antidepressant-like activity. Moreover, in learning and memory experimental models, the treated animals reversed scopolamine-induced amnesic effects as evident from improved step-through latencies (P < 0.05 vs. scopolamine; passive avoidance test), spontaneous alternation behavior (P < 0.05 vs. scopolamine; Y-maze test), discrimination index (P < 0.05 vs. scopolamine; novel object recognition test), and escape latencies (P < 0.05 vs. scopolamine; Morris water maze). Biochemical studies of isolated brains from treated rats demonstrated significantly elevated levels of superoxide dismutase and glutathione peroxidase (P < 0.05), whereas levels of acetylcholinesterase and malondialdehyde level (P < 0.05) were reduced, indicating its potential to reduce oxidative damage in the brain and modulation with the cholinergic system. The outcomes of studies support the benefits of phytoconstituents possessed by G. asiatica fruit in the amelioration of neurological disorders that could be due to their antioxidative capacity or due to interaction with GABAergic, serotonergic, and cholinergic systems in the brain.

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In view of anxiolytic, antidepressant and memory strengthen properties of Allium cepa (AC; onion) bulb in various investigations; we aimed to evaluate the useful effects of onion on single immobilization stress -induced biochemical and behavioral changes. Mice in test group were treated with AC powder (200 mg/kg/day), dissolved in water, while the control group were received drinking water for 14 days. After 14 days control and AC treated mice were further divided into unstressed and stressed groups. Animals in the stressed group were subjected to immobilization stress for 2 h. 24-h after the immobilization stress, behavioral activities were monitored. Immobilization stress-induced an anxiogenic behavior in mice subjected to elevated plus maze test (EPM) and light dark activity test (LDA). 2-h immobilization stress-induced depressive behavior in animals measured by forced swim test (FST). Administration of AC attenuated the immobilization stress-induced behavioral deficits. Highest memory performance was observed in stressed mice that were pre-treated with AC in Morris water maze (MWM). Brain lipid peroxidation, antioxidant enzymes (SOD, CAT, GPx) and acetylcholinesterase (AChE) activities were also estimated. Present study suggests a role of antioxidant enzymes in the attenuation of 2-h stress induced anxiety and depression, and enhanced cognitive function as well by AC. The findings therefore suggest that supplementation of AC may be beneficial in the treatment of anxiety, depression and enhancement of memory function.

The determination of the seven elements was performed by Perkin Elmer Atomic Absorption (AA) spectrophotometer. The present study highlights the importance of seven heavy metals residual concentration including Cd, Cr, Cu, Fe, Mn, Ni and Zn in milk of Camel, Cattle, Buffalo, Sheep and Goat from various areas of Khyber Pakhtunkhwa (KPK), Pakistan. It revealed that milk of camel comprising of high levels of Zn (5.150 ± 0.021 mg/kg), Mn (0.094 ± 0.003 mg/kg) and Fe (1.580 ± 0.530 mg/kg) with a definite correlation. In the milk of buffalo, high concentration of noxious heavy metals including Cu (0.223 ± 0.010 mg/kg) and Cd (0.117 ± 0.086 mg/kg) were found whereas in goat milk, high Ni (1.152 ± 0.045 mg/kg) and Cr (1.152 ± 0.045 mg/kg) was observed and detected. The analysis showed that camel and buffalo have similar high concentration of heavy metals. Overall results showed that milk of cattle shows higher concentration of Zn, Mn and Fe along with Buffalo.

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