E. longifolia is attracting interest due to its pharmacological properties and pro-vitality effects. In this study, an online SPE-LC approach using polystyrene divinyl benzene (PSDVB) and C18 columns was developed in obtaining chromatographic fingerprints of E. longifolia. E. longifolia root samples were extracted using pressurized liquid extraction (PLE) technique prior to online SPE-LC. The effects of mobile phase compositions and column switching time on the chromatographic fingerprint were optimized. Validation of the developed method was studied based on eurycomanone. Linearity was in the range of 5 to 50 µg¨mL´1 (r 2 = 0.997) with 3.2% relative standard deviation of peak area. The developed method was used to analyze 14 E. longifolia root samples and 10 products (capsules). Selected chemometric techniques: cluster analysis (CA), discriminant analysis (DA), and principal component analysis (PCA) were applied to the fingerprint datasets of 37 selected peaks to evaluate the ability of the chromatographic fingerprint in classifying quality of E. longifolia. Three groups were obtained using CA. DA yielded 100% correlation coefficient with 19 discriminant compounds. Using PCA, E. longifolia root samples were clearly discriminated from the products. This study showed that the developed online SPE-LC method was able to provide comprehensive evaluation of E. longifolia samples for quality control purposes.
Pressurised liquid extraction (PLE) method was utilised to extract eurycomanone from Tongkat Ali root (Eurycoma longifolia) using water as extraction solvent. Optimisation of PLE operating parameters was conducted using three-factor response surface experimental design to evaluate the interactive effects of temperature (90 -150 ºC), extraction time (10 -30 min) and pressure (850 -1700 psi) on the yield of eurycomanone. Online solid phase extraction -high performance liquid chromatography (SPE-HPLC) with a diode array detector (DAD) was used for the separation and detection of this compound. Extraction temperature was found significant in increasing the yield of eurycomanone. Based on maximum yield of eurycomanone, the optimum operating extraction conditions for PLE were set up at temperature (106 °C), pressure (870 psi) and static time (30 min). The amount of eurycomanone from Tongkat Ali root samples of various sources was analysed using the optimised PLE conditions. Keywords: pressurised liquid extraction, Eurycoma longifolia, eurycomanone, solid phase extraction -liquid chromatography Abstrak Kaedah pengekstrakan cecair tekanan tinggi (PLE) telah digunakan untuk mengekstrak eurikomanon daripada akar Tongkat Ali (Eurycoma longifolia) menggunakan air sebagai pelarut. Pengoptimuman parameter operasi PLE dijalankan dengan menggunakan reka bentuk eksperimen gerak balas permukaan tiga faktor untuk menilai kesan interaktif suhu (90 -150 °C), masa pengekstrakan (10 -30 min) dan tekanan (850 -1700 psi) ke atas hasil eurikomanon. Pengekstrakan fasa pepejalkromatografi cecair tekanan tinggi (SPE-HPLC) dengan pengesan diod (DAD) telah digunakan untuk pemisahan dan pengesanan sebatian ini. Suhu pengekstrakan didapati penting dalam meningkatkan hasil eurikomanon. Berdasarkan hasil maksimum eurikomanon, pengkstrakan optimum untuk PLE, ialah suhu (106 °C), tekanan (870 psi) dan masa static (30 minit). Amaun eurikomanon daripada pelbagai sampel akar Tongkat Ali telah dianalisis dengan menggunakan keadaan PLE yang telah dioptimumkan.
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