AN EXPERIMENTAL DESIGN METHOD FOR THE EXTRACTION OF EURYCOMANONE FROM TONGKAT ALI (Eurycoma longifolia) ROOTS USING PRESSURISED LIQUID EXTRACTION (PLE)

Osman, Rozita; Saim, Norashikin; Saaid, Mardiana; Zaini, Nor Nasriah

doi:10.17576/mjas-2016-2002-17

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Both markers for their utilization in routine quantitative authentication require pure isolates for eurycomanone and protein of Marker A. Eurycomanone can be purchased easily from many reputable suppliers, though priced exorbitantly for a few milligrams. Some workers have managed to reduce the extraction time to less than 20 min compared to 16 h using the conventional distillation technique 28 . For the marker A protein, the challenge resides not with its purification but the tedious 2DE procedures.…”

Section: Discussionmentioning

confidence: 99%

Marker to Authenticate <i>Eurycoma Longifolia</i> (Tongkat Ali) Containing Aphrodisiac Herbal Products

Vejayan¹,

Mohamed²,

Zulkifli³

et al. 2018

Current Science

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The benefits of Eurycoma longifolia (Tongkat Ali, TA) for its aphrodisiac capabilities are well known and many products are marketed worldwide. Due to its popularity, the plant is being abused for promoting fake products. Therefore, there is a need for better testing of the markers required by authorities and responsible manufacturers. A low-molecular-weight protein has been studied for developing it as a testing marker. Two dimensional electrophoresis (2DE) (four spots were observed) was used for positive detection of proteins in an aqueous extract of TA root and the pronounced separation of a Coomassie-stained spot, subsequently referred to as Marker A. Consecutive chromatographic separations of the aqueous extract of TA led to the isolation of pure protein from Marker A. When this marker was used to test 46 TA-based products randomly selected from markets worldwide, in 20 of them, the results were found to be comparable to those obtained using the organic eurycomanone marker. The ranking of products from the highest quantity to the lowest observed to be ordered differently if compared for both markers. This is an expected outcome because Marker A was measured for its protein content and eurycomanone for its organic molecule. Marker A detection using 2DE is shown to be a useful tool to test products supplemented with E. longifolia root.

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Section: Discussionmentioning

confidence: 99%

Marker to Authenticate <i>Eurycoma Longifolia</i> (Tongkat Ali) Containing Aphrodisiac Herbal Products

Vejayan¹,

Mohamed²,

Zulkifli³

et al. 2018

Current Science

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“…Dried root of E. longifolia was weighed (2.0 g) and mixed with an equal amount of diatomaceous earth, then transferred to a 34 mL PLE stainless steel extraction cell. Extraction was carried out at 100°C and 1500 psi for 30 min [31]. E. longifolia product (1.1 g) was dissolved in 15 mL of hot water (~80°C) and filtered using filter paper prior to online SPE-LC analysis.…”

Section: Methodsmentioning

confidence: 99%

Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)

et al. 2016

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E. longifolia is attracting interest due to its pharmacological properties and pro-vitality effects. In this study, an online SPE-LC approach using polystyrene divinyl benzene (PSDVB) and C18 columns was developed in obtaining chromatographic fingerprints of E. longifolia. E. longifolia root samples were extracted using pressurized liquid extraction (PLE) technique prior to online SPE-LC. The effects of mobile phase compositions and column switching time on the chromatographic fingerprint were optimized. Validation of the developed method was studied based on eurycomanone. Linearity was in the range of 5 to 50 µg¨mL´1 (r 2 = 0.997) with 3.2% relative standard deviation of peak area. The developed method was used to analyze 14 E. longifolia root samples and 10 products (capsules). Selected chemometric techniques: cluster analysis (CA), discriminant analysis (DA), and principal component analysis (PCA) were applied to the fingerprint datasets of 37 selected peaks to evaluate the ability of the chromatographic fingerprint in classifying quality of E. longifolia. Three groups were obtained using CA. DA yielded 100% correlation coefficient with 19 discriminant compounds. Using PCA, E. longifolia root samples were clearly discriminated from the products. This study showed that the developed online SPE-LC method was able to provide comprehensive evaluation of E. longifolia samples for quality control purposes.

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Efficient extraction of quassinoids and alkaloids from Eurycoma longifolia Jack roots using natural deep eutectic solvents and microwave-assisted extraction

Sitthisak,

Nisoa,

Chunglok

et al. 2024

Microchemical Journal

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AN EXPERIMENTAL DESIGN METHOD FOR THE EXTRACTION OF EURYCOMANONE FROM TONGKAT ALI (Eurycoma longifolia) ROOTS USING PRESSURISED LIQUID EXTRACTION (PLE)

Cited by 3 publications

References 20 publications

Marker to Authenticate <i>Eurycoma Longifolia</i> (Tongkat Ali) Containing Aphrodisiac Herbal Products

Marker to Authenticate <i>Eurycoma Longifolia</i> (Tongkat Ali) Containing Aphrodisiac Herbal Products

Development of Chromatographic Fingerprints of Eurycoma longifolia (Tongkat Ali) Roots Using Online Solid Phase Extraction-Liquid Chromatography (SPE-LC)

Efficient extraction of quassinoids and alkaloids from Eurycoma longifolia Jack roots using natural deep eutectic solvents and microwave-assisted extraction

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