In the study, it is aimed to research the likely role of visfatin in hyperthyroid and subclinical hyperthyroid status related to dislipidemia, after determining the visfatin and PON-1 levels thirty healthy individuals were enrolled in this study as control group and thirty hyperthyroidism patient's with dyslipidemia they were treated with. The subjects aged ranged (30-50) years and with BMI (25-29) kg/m 2 . Serum was used in determination of lipid profile total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), thyroid threonine (T3) and thyroxin (T4), thyroid stimulating hormone (TSH), visfatin and paraoxonase-1 (PON-1). The results showed significant increase in T3, T4, TC, TG, LDL and VLDL in patient group when comparing to control group. While significant decrease was found in TSH and HDL in patient group when comparing to control group. The results also represented significant increase in visfatin levels in patient group comparing to control group. While there are significant reducation in PON-1 levels in patient group comparing to control group. The conclusion could be drown from this study that visfatin and PON-1 effected hyperthyroidism patient's with dyslipidemia All the patients in hyperthyroid group were heterozygotes for Familial Hypercholesterolemia is a disorder that is passed down through families. It causes LDL (bad) cholesterol level to be very high. The condition begins at birth and can cause heart attacks at an early age.
The study aimed to estimate the role of glucagon-like peptide-1 (GLP-1) and visfatin as a novel pro inflammatory marker in Rheumatoid Arthritis (RA ) according to the activity scores of disease to assess the possibility of introducing glucagon-like peptide-1 and visfatin in the diagnosis and monitoring of RA patients and to found the correlation of visfatin level with GLP-1 and AIP in patients prone to atherosclerosis ,fifty healthy individuals as control group (G1) and fifty rheumatoid arthritis (RA) patients (G2) were enrolled in this study with middle age ranged (30 – 40) years and BMI ≥24 kg/m2. ESR ,RF, lipid profile, CRP,insulin, visfatin and GLP-1were determined. Results in table (1) revealed highly significant increase in ESR,RF,TC,TG,LDL,VLDL and AIP level in rheumatoid arthritis (G2) comparing to (G1) while significant decrease noticed in (G2) group comparing to (G1). Results in table(2) showed highly significant increase in insulin ,CRP, visfatin and GLP-1 level Results in table(3) illustrated correlation relation data and results revealed highly significant ,were r=0.035 for GLP-1 with visfatin for (G1), and highly significant , r= -0.089 for (G2) as shown in figure(1-A) and(1-B), the result also highly significant were r= -0.183 for GLP-1 with CRP for control group and significant ,r= 0.043 for GLP-1 with CRP for RA group as shown in figure(2-A) and(2-B), and significant, r= -0.056 for GLP-1with AIP for G1 and no significant, were r= 0.042 for GLP-1 with AIP for G2 as shown in figure (3-A)and (3-B). conclusion that summarized from this study is glucagon-like peptide-1 and visfatin plays a role in RA pathogenesis that can be considered marker in RA . and may be used as a drug in future for RA patients.
The aim of this study purification of Leucine aminopeptidase (LAP) from hypothyroidism patients sera and its relation to thyroid hormones, lipid peroxidation levels of subclinical hypothyroidism and hypothyroidism patients, blood samples were collected from (50) healthy subject and (100) patients, consisted of fifty with hypothyroidism and fifty with subclinical hypothyroidism.. The purification is done by addition of ammonium sulfate, dialysis, anion- exchange chromatography and size-exclusion chromatography. FT3, FT4, TSH, LAP, peroxynitrite, malondialdehyde were determined. Results showed the precipitate and concentrated protein appeared five peaks in ion exchange column, LAP activity located in the first and second protein peak, also, results showed single peak for both first and second peaks after eluted in gel filtration chromatography following steps by using SDS-polyacrylamide gel electrophoresis. From current study, it is concluded hypothyroidism (LAP) have two isoenzymes, also, concluded a highly significant increase in LAP, MDA and peroxynitrite levels in the subclinical hypothyroidism and hypothyroidism patients when compared to the healthy subject, and their correlation with FT3, FT4, TSH in the patient group, when compared by the control group that indicate these parameters could be play an effect role in these diseases.
The new, standard molecular biologic system for duplicating DNA enzymatically devoid of employing a living organism, like E. coli or yeast, represents polymerases chain reaction (PCR). This technology allows an exponential intensification of a minor quantity of DNA molecule several times. Analysis can be straightforward with more DNA available. A thermal heat cycler performs a polymerization chain reaction that involves repeated cycles of heating and cooling the reactant tubes at the desired temperature for each reaction step. A heated deck is positioned on the upper reaction tube to avoid evaporating the reaction mixture (normally volumes range from 15 to 100 l per tube), or an oil layer can be placed on a reaction mixture surface. The amplified DNA fragment is determined based on selecting primers in addition to the starting and end of the DNA fragment. The primers stand for short, artificial DNA stripes, no higher than fifty (typically 18-25bp) nucleotides have been based on a starting and ending of DNA fragment to be amplified. DNA-polymerase connects and starts a new DNA strand synthesis The PCR products can be visualized by dual foremost methods: (1) staining of the product of DNA amplified by a chemical dye like bromide ethidium, or (2) marking of fluorescent dyes (fluorophores) PCR primers or nucleotides before amplification of PCRs. PCR offers some benefits. First, it is a simple method of understanding and using and quick results. It has an extremely sensitive technology with the potential for sequencing, cloning, and analyzing millions or milliards of copies of a particular product.
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