Background: Most of one-bottle self-etch dental adhesives or so-called "all-in-one" system contains a mixture of hydrophilic and hydrophobic components that are dissolved in an organic solvent. This variability between the adhesives would affect their interaction with tooth structures. Flaw in this interaction, represented by poor dentin hybridization might affect the treatment success. Thus, the objective of the study was to compare between two different all-in-one self-etch adhesives, by examining the nanoleakage expression at the dentin-resin interface.Methodology: Two all-in-one self-etch adhesives; water-based Tetric-N bond adhesive (TN; Ivoclar/Vivadent) and ethanol/water-based Tri-S bond plus (TS; Kuraray Noritake) adhesive, were used for bonding resin composite to human dentin. In order to prepare the specimen for nanoleakage evaluation, all specimens were sectioned, coated with nail-varnish and subjected to ammoniacal silver nitrate solution followed by developing solution. Then, images were taken under scanning electron microscope (SEM) and investigated by energy-dispersive X-ray spectroscopy (EDS) to trace the silver grains at the dentin-resin interface.Result: Unlike in TS group that did not show silver deposition throughout the bonded area, the TN group showed heavy silver deposition along the hybrid layer as well as throughout the adhesive layer using SEM/EDS. Conclusion:It can be concluded that water-based all-in-one adhesive would adversely affect the longevity of polymeric dental restoration as it showed heavy silver deposition along the dentin-resin interface.
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