Comparative SEM Observation of Silver-Nitrate at Resin-Dentin Interface: Nanoleakage Study

Abstract

Background: Most of one-bottle self-etch dental adhesives or so-called "all-in-one" system contains a mixture of hydrophilic and hydrophobic components that are dissolved in an organic solvent. This variability between the adhesives would affect their interaction with tooth structures. Flaw in this interaction, represented by poor dentin hybridization might affect the treatment success. Thus, the objective of the study was to compare between two different all-in-one self-etch adhesives, by examining the nanoleakage expression at the dentin-resin interface.Methodology: Two all-in-one self-etch adhesives; water-based Tetric-N bond adhesive (TN; Ivoclar/Vivadent) and ethanol/water-based Tri-S bond plus (TS; Kuraray Noritake) adhesive, were used for bonding resin composite to human dentin. In order to prepare the specimen for nanoleakage evaluation, all specimens were sectioned, coated with nail-varnish and subjected to ammoniacal silver nitrate solution followed by developing solution. Then, images were taken under scanning electron microscope (SEM) and investigated by energy-dispersive X-ray spectroscopy (EDS) to trace the silver grains at the dentin-resin interface.Result: Unlike in TS group that did not show silver deposition throughout the bonded area, the TN group showed heavy silver deposition along the hybrid layer as well as throughout the adhesive layer using SEM/EDS. Conclusion:It can be concluded that water-based all-in-one adhesive would adversely affect the longevity of polymeric dental restoration as it showed heavy silver deposition along the dentin-resin interface.

Evaluation of the adaptation of dental polymers using cross-polarization optical coherence tomography

“…The absence of HEMA would enhance the bonding ability to dentin by reducing the water uptake and consequently less hydrolytic degradation [48,49]. However, the high-water contents in this adhesive may adversely affect complete water elimination from the demineralized dentin during the airdrying step, as the water vapor pressure is relatively low in comparison to acetone and ethanol [15,50]. These prospects are coinciding with other reports on HEMA-free and water-based adhesives, which demonstrated water-tree and nano-leakage within the hybrid layer that were attributed to high water inclusion and compromised polymerization [51,52].…”

Evaluation of Matrix Metalloproteinases Biodegradation Inhibition Protocols on Resin-Dentine Interfacial Characteristics (An In Vitro Comparative Study)

“…Examination was done for prepared slabs at 1000× magnification with scanning electron microscope (10) . Analyzing the distribution of metallic silver particles at the dentine-resin interface was done using the digital image software Fiji (https://imagej.net/Fiji) (11) , in a selected area (height × width = 21 × 250 µm) on each image. The Otsu method was used for the SEM images to convert into binary images; thus, the silver area was identified as black pixels on a white background, and the percentage of silver penetration at the interfacial zone was calculated (10,12) .…”