Balanophora subcupularis P.C. Tam and Balanophora tobiracola Makino are two new recently recorded species of the genus Balanophora J.R. Forst and G. Forst in Viet Nam. In the present work, we investigated the phytochemical component, in vitro xanthine oxidase (XO) inhibitory activity of methanol extracts, fractions (n-hexane and ethyl acetate) and water residue of these two species. The Thin layer chromatography (TLC) of the methanol extracts and fractions of the two species were also done. The Ethyl acetate fractions of the two species showed the best XO inhibitory activity with IC50 values of 48.41 ± 1.56 µg/mL and 11.87 ± 1.28 µg/mL for B. subcupularis and B. tobiracola, respectively. The results show that the ethyl acetate fractions of the two Balanophora species may serve as a potential source of bioactive constituent for use in the treatment of gout.
The objective of this study is to determine the morphological characteristics of flowers and pollen, preliminary phytochemical evaluation and thin layer chromatography of five species of genus Balanophora J.R. & G. Forst collected in Viet Nam: Balanophora fungosa J.R. & G. Forst ssp. indica (Arn.) Hansen var. indica, Balanophora fungosa J.R. & G. Forst ssp. indica (Arn.) Hansen var. globosa (Jungh.) Hansen, Balanophora latisepala (Tiegh) Lecome, Balanophora laxiflora Hemsl, Balanophora subcupularis P.C. Tam. The morphological characteristics of male flowers, female flowers and spadicles, pollen grain of five species were described. Results of preliminary phytochemical evaluation of ethanol extracts of five species showed that all of five species have tannins, flavonoids, coumarins, sterols. Thin layer chromatography of ethanol extracts of five species were developed and base on TLC chromatogram, it is easy to differentiate these species.
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