Radiofrequency radiations (RFRs) emitted by mobile phone base stations have raised concerns on its adverse impact on humans residing in the vicinity of mobile phone base stations. Therefore, the present study was envisaged to evaluate the effect of RFR on the DNA damage and antioxidant status in cultured human peripheral blood lymphocytes (HPBLs) of individuals residing in the vicinity of mobile phone base stations and comparing it with healthy controls. The study groups matched for various demographic data including age, gender, dietary pattern, smoking habit, alcohol consumption, duration of mobile phone use and average daily mobile phone use. The RF power density of the exposed individuals was significantly higher (p < 0.0001) when compared to the control group. The HPBLs were cultured and the DNA damage was assessed by cytokinesis blocked micronucleus (MN) assay in the binucleate lymphocytes. The analyses of data from the exposed group (n = 40), residing within a perimeter of 80 m of mobile base stations, showed significantly (p < 0.0001) higher frequency of micronuclei when compared to the control group, residing 300 m away from the mobile base station/s. The analysis of various antioxidants in the plasma of exposed individuals revealed a significant attrition in glutathione (GSH) concentration (p < 0.01), activities of catalase (CAT) (p < 0.001) and superoxide dismutase (SOD) (p < 0.001) and rise in lipid peroxidation (LOO) when compared to controls. Multiple linear regression analyses revealed a significant association among reduced GSH concentration (p < 0.05), CAT (p < 0.001) and SOD (p < 0.001) activities and elevated MN frequency (p < 0.001) and LOO (p < 0.001) with increasing RF power density.
The study aims to evaluate the anticancer activity of Callicarpa arborea in A549 cancer cells. Fresh non-infected leaves of Callicarpa arborea were collected from Serkawn, Lunglei District, Mizoram and various solvents were used for Soxhlet extraction at their respective boiling points. The extracts were concentrated and the anticancer activity was tested in the human lung cancer cell line A549 using MTT and clonogenic assays. The effect of C. arborea on the antioxidant system was also assessed by measuring the levels of glutathione, glutathione s-transferases, superoxide dismutase as well as lipid peroxidation levels following standard protocols. Among the various solvent extracts of C. arborea, only chloroform extract showed significant cytotoxicity, and inhibited cell proliferation and survival against the A549 cancer cells. The chloroform extract of C. arborea induced cell death in A549 cells in a dose and time dependent manner with an IC of 52.8 20.4 50-1-1 µgml and µgml at 24 hr and 48 hr, respectively. The clonogenic assay showed that the chloroform extract was able to inhibit cell proliferation in the A549 cells and the inhibition increased with increase in dose. The chloroform extract also alleviated the levels and activities of antioxidants glutathione, glutathione-s-transferase and superoxide dismutase, while elevating the lipid peroxidation level in the A549 cells. The study shows that Callicarpa arborea possess both cytotoxic and anti-proliferative properties against the human lung cancer cell line A549. Callicarpa arborea is a potential candidate as a new anti-cancer agent and warrants further investigation. Anticancer agent, Antioxidants, Callicarpa arborea, Cell proliferation, Cytotoxicity Anticancer activity of Callicarpa arborea Roxb. extracts against Type-II human lung adenocarcinoma cell line, A549
To investigate the effects of low doses of X-ray exposure on chromosomal damage and antioxidants level in cultured human peripheral blood. Blood samples collected from healthy young volunteers (male and female) in sterile heparinized tubes were irradiated at 97 kVp with 300 mAs using X-rays machine. In-vitro irradiation of whole blood was performed at different doses (0, 25, 50, 100, 200 and 300 mGy) with an average dose-1 rate of 19.6 mGysec. After irradiation, the lymphocytes were collected and cultured for 72 hr. Micronuclei (MN) assay was carried out following the standard protocol for the assessment of chromosomal damage. The level of glutathione (GSH), activities of glutathione-s-transferase (GST) and superoxide dismutase (SOD) were estimated from the blood plasma. Malondialdehyde (MDA) content was also estimated to determine oxidative stress after low-dose irradiation. 2 A significant positive correlation (r =0.98, p <0.001) was observed between total MN frequency and irradiation dose in human peripheral blood lymphocytes. Irradiation of blood also caused significant decrease in GSH level and GST activities with increased in irradiation dose. Significant reduction in SOD activity was observed only at doses ≥ 100 mGy. Induction of oxidative stress in human blood due to irradiation was clearly evident from enhanced MDA content. This study indicates that exposure to ionizing radiation less than 100 mGy can cause genetic damage and induce oxidative stress. F u r t h e r m o r e , t h e r e s u l t s suggested that detection of genetic damage using MN assay is sensitive enough at a lower dose in contrast to IAEA manual where the detection limit is Antioxidants, Human lymphocytes, Lipid peroxidation, Micronucleus, X-ray only 0.2-0.3 Gy. Effects of low-dose irradiation on chromosomal damage and oxidative stress in cultured human peripheral blood
No abstract
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.