INTRODUCTION: Keeping quality of meat and meat related food hazard relates to microbes present in the meat during processing or storage. The poultry slaughtered and dressed under Chitwan conditions carrying high initial contamination would be present in meat as inherent contamination in the finished products. MATERIALS AND METHODS: This cross-sectional study included 26 fresh broiler meat samples from registered retail shops. The samples were subjected to bacteriological analysis such as total viable count (TVC), total enterobacteriaceae count (TEC), total coliform count (TCC) and total staphylococcal count (TSC). Also, the samples were analyzed for the detection of Salmonella spp. and identified by different biochemical tests. RESULTS: The mean counts in log10±SE colony forming units per gram (cfu/g) for Bharatpur, Ratnanagar and Institute of Agriculture and Animal Science (IAAS) vicinity were obtained as 11.1±0.3, 11.5±0.3 and 12.2±0.5 TVC; 8.5±0.2, 9.2±0.3 and 10.2±0.4 TEC; 6.5±0.3, 7.6±0.3 and 8.4±0.5 TCC; 6.5±0.2, 6.8±0.3 and 7.7±0.4 TSC respectively. No samples were found to be within the permissible limits given by different agencies. Prevalence of Salmonella spp. in retail broiler meat in Chitwan was found 46.2%. CONCLUSION: The retail broiler meat samples from the locations contain high count of bacteria suggesting deplorable state of hygienic and sanitary practices. The presence of Salmonella and Staphyloccus aureus organisms over the permissible limits are of special concern because these account for potential food borne intoxication. So, the need for microbial assessment of fresh meats can be emphasized. DOI: http://dx.doi.org/10.3126/ijim.v2i3.8671   Int J Infect Microbiol 2013;2(3):99-104

A survey was conducted in November 2015 in one of the pocket area of large cardamom production in Teharthum District, eastern Nepal with aim to investigate the status of cardamom enterprises. The parameters used were cardamom production area, type of manure used, drying facilities, technical skills of farmers, market channels and variable cost etc. We purposively selected 30 cardamom producers and stakeholders for interview pre-designed questionnaires. The result showed that average area, production and productivity of large cardamom per household were 0.86 ha, 200 kg and 232 kg.ha -1 , respectively, with the average farming experience of 22 years. It was revealed that 13% farmers used farmyard organic manure, the use of 1.5 kg/plant farmyard manure might produce 28.5% higher yield cardamom compared to without using any manure or fertilizers. It was also revealed among the responded only 7% had received improved drying machine from District Agriculture Development Office (DADO) at50% subsidy, while only 23% of farmers received training and technical services from DADO. The study showed that per hectare average total cost of large cardamom production, selling price and gross revenue were NRs. 2,36,705 ($2255), NRs. 5,50,305 ($5240) and NRs. 3,13,600 ($2985), respectively, with benefit/cost (B/C) ratio of 2 after the completion of gestation period of 4 years. Our survey showed that predominant marketing approach was by direct sell to the traders located at district headquarter. The productivity of large cardamom was influenced by various factors, such as nearly 75.2% of the variation in productivity was explained by the number of active family members, farming period, area, intercultural operations, variable cost and depreciated fixed cost.

The study was carried out to examine the optimum dose of Nitrogen on Broccoli in Institute of Agriculture and Animal Science, Lamjung Campus during Sept, 2015- Jan, 2016. Six treatment Viz. 50, 100,150,200, 250, 300 kg/ha of Nitrogen with same level of P and k (160:100 kg/ha) and FYM (20 t/ha) were used in a Randomized Complete Block Design with three replications. Effect of treatments on plant height, leaf area and head weight plant-1, yield ha-1 were significant and other parameters (leaf number, SPAD reading, plant spreading) were not affected by the treatments. Treatment that received 300 kg N ha-1 gave the maximum yield ha-1 (14.77 ton) which was followed by treatment that received 250 kg N ha-1 (10.60 ton), 200 kg N ha-1 (8.11 ton), 150 kg N ha-1 (7.432 ton), 100 kg N ha-1 (5.96 ton) and 50 kg ha-1 (4.24 ton). Treatments 250 kg N ha-1, 200 kg N ha-1, 150 kg N ha-1 and 100 kg N ha-1 were statistically at par while 50 kg produced the least yield ha-1. Application of 300 kg N ha-1 gave the higher result with respect to plant height (17.75 cm), leaf area (699.32 cm2), leaf number (7.77), SPAD reading (68.71), plant spreading (70.21 cm), compared to other treatments while minimum result obtained from application of 50 kg N ha-1. Hence, we can conclude that the total head production increased with increased N Int. J. Appl. Sci. Biotechnol. Vol 6(3): 270-273

An investigation was carried out to study the performance of mandarin grafts during 2017-18 at Banepa, Kavre to standardize suitable grafting time for two mandarin varieties. The experiment was conducted in two factorial Completely Randomized Design (CRD) with 10 treatments and three replications. The treatments consisted of combinations of five grafting dates (25 th November, 10 th December, 25 th December, 10 th January, and 25 th January) and two mandarin varieties (Dhankuta Local and Ota Ponkan). Scion collected from mother plants of respective mandarin varieties were grafted onto a trifoliate orange rootstock by side veneer method in the screen house. The results showed that grafting dates significantly affect the graft success and subsequent growth of grafted sapling of mandarin varieties. The highest graft success (100%) was observed on the grafts prepared in January. The sapling height (47.58 ± 1.93 cm), leaf area (32.74 ± 2.98 cm 2 ), number of primary branches per sapling (3.16 ± 0.20) and increased in diameter above union (17.78 ± 1.47%) were recorded maximum on the grafts prepared on 25 th January while the number of leaves per sapling (26.81 ± 1.84) was found superior for 10 th January grafted sapling. Most of the important parameters viz., graft success percent, sapling height, and leaf area were found nonsignificant for varieties but the number of leaves per sapling (23.99 ± 1.51), number of primary branches per sapling (2.72 ± 0.21), increased in diameter above union (16.24 ± 0.88%) and at union (13.72 ± 1.54%) was found maximum for Ota Ponkan. Therefore, the study revealed that late January was the most suitable time of grafting for both varieties of mandarin under Kavre, Nepal conditions.

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