Context: Physical exercise with maximum intensity to fatigue can cause impaired immunity. Gastrocnemius muscle damage is found in eccentric activities in sports. Vitis gracilis has been proven as a healing agent for degenerative diseases. Aims: To determine whether the V. gracilis Wall extract can recovery of gastrocnemius muscle cells of Mus musculus through reduced inflammatory cells and cytochrome c expression. Methods: The studies turned into performed in 5 groups of remedies withinside the study. The remedy groups consisted of T1: Negative control (no treatment), T2: Positive control (Swim Until the First Sink/SUFS) + Vit. C 0.2 mg/kg BW, T3: SUFS + 50 g/kg BW of V. gracilis, T4: SUFS + 75 g/kg BW of V. gracilis and T5: SUFS + 100 g/kg BW of V. gracilis. Mice were dissected and then the leg were taken for analysis of gastrocnemius muscle cells in immunohistochemistry. Results: There was a significant difference (p<0.01) using the ANOVA one-way test on inflammatory cells in gastronomeus muscle tissue of Mus musculus and cytochrome c expression. V. gracilis 100 mg/kg BW can repair and reduce inflammatory cells in the gastronomic muscle tissue. The expression of cytochrome c protein becomes weaker when it is increased. Conclusions: V. gracilis can recover gastrocnemius muscle cells of Mus musculus through reduced inflammatory cells and cytochrome c expression because of swim exercise.
Background and Objective: Herbs have the potential to be used in molecular therapy to treat inflammation and apoptosis. It has been demonstrated that the Indonesian native Vitris gracilis inhibits cytochrome c as a precursor to apoptosis. When body tissues are injured, infected with bacteria, exposed to toxins or exposed to heat that causes cell death, inflammation occurs. The current study aimed to look for the effect of Vitis gracilis extract in the lung histology of mice. Materials and Methods: Five treatment groups were used for the experiments, which were conducted inside the study. The T1 is the negative control, T2 is swimming mice with 0.2 mg kgG 1 b.wt., of vitamin C, T3 is swimming mice with 50 mg kgG 1 b.wt., of Vitis gracilis, T4 is swimming mice with 75 mg kgG 1 b.wt., of Vitis gracilis and T5 is swimming mice with 100 mg kgG 1 b.wt., of Vitis gracilis. The dislocation procedure was used to dissect mice, ketamine was administered before dissection and the lungs were removed for TUNEL assay examination. Results: There were no significant differences in inflammatory cells or index-positive apoptotic cells between the T1, T2 and T5 groups (p>0.05). The T3 group had the highest value, while the T1 group had the lowest. The highest dose of Vitis gracilis reduced lung cell inflammation while also improving histological structure, resulting in intact, nucleated and complete alveolar membranes with proportional endothelial cells. Conclusion: Vitis gracilis 100 mg kgG 1 b.wt., can repair and reduce inflammation in lung tissue. As a result, the higher the dose of Vitis gracilis, the less cell apoptosis occurs in the lungs.
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