Effect of Vitis gracilis Wall (gagatan harimau) in the recovery of gastrocnemius muscle cells and cytochrome c expression of Mus musculus

Abstract

Context: Physical exercise with maximum intensity to fatigue can cause impaired immunity. Gastrocnemius muscle damage is found in eccentric activities in sports. Vitis gracilis has been proven as a healing agent for degenerative diseases. Aims: To determine whether the V. gracilis Wall extract can recovery of gastrocnemius muscle cells of Mus musculus through reduced inflammatory cells and cytochrome c expression. Methods: The studies turned into performed in 5 groups of remedies withinside the study. The remedy groups consisted of T1: Negative control (no treatment), T2: Positive control (Swim Until the First Sink/SUFS) + Vit. C 0.2 mg/kg BW, T3: SUFS + 50 g/kg BW of V. gracilis, T4: SUFS + 75 g/kg BW of V. gracilis and T5: SUFS + 100 g/kg BW of V. gracilis. Mice were dissected and then the leg were taken for analysis of gastrocnemius muscle cells in immunohistochemistry. Results: There was a significant difference (p<0.01) using the ANOVA one-way test on inflammatory cells in gastronomeus muscle tissue of Mus musculus and cytochrome c expression. V. gracilis 100 mg/kg BW can repair and reduce inflammatory cells in the gastronomic muscle tissue. The expression of cytochrome c protein becomes weaker when it is increased. Conclusions: V. gracilis can recover gastrocnemius muscle cells of Mus musculus through reduced inflammatory cells and cytochrome c expression because of swim exercise.

Analysis of Apoptotic Cells and Lung Inflammation after Given by Vitis gracilis

“…The leaves of Vitis gracilis contain alkaloids, flavonoids, saponins, tannins and glycosides 2 . As medicinal ingredients, phenols, organic acids, glycosides, amino acids, flavonoids and other chemical as antioxidant constituents of Vitis gracilis can be developed 2,3 . Another study using other herbs showed that dietary curcumin ameliorated radiation-induced pulmonary fibrosis and increased survival in mice without interfering with radiation-induced tumor cell killing 12,13 .…”

“…The cells of the lungsʼ alveoli are very thin (0.2-0.5 m) and arranged in such a way that alveolar sacs form 20 . When stretched, the entire lung alveoli cells reach (a width of 70-80 m 2 ). An interalveolar wall (septum) connects the alveolar sacs and is made up of two layers: Simple squamous epithelium and connective tissue 20,21 .…”

“…Vitis gracilis is a traditional medicinal plant of the Karo people of North Sumatra that works well as a stamina booster 1 . According to the results of phytochemical screening, Vitis gracilis leaves include glycosides, alkaloids, saponins, tannins and flavonoids 2 . It is possible to create pharmaceutical compounds from the chemical components of Vitis gracilis, including its flavonoids, glycosides, phenols, amino acids and organic acids [1][2][3] .…”

“…According to the results of phytochemical screening, Vitis gracilis leaves include glycosides, alkaloids, saponins, tannins and flavonoids 2 . It is possible to create pharmaceutical compounds from the chemical components of Vitis gracilis, including its flavonoids, glycosides, phenols, amino acids and organic acids [1][2][3] . This plant can regenerate the gastrocnemius muscle cells in Mus musculus through a reduction in inflammatory cells and cytochrome c expression brought on by swimming activity 2 .…”