In this study, we report the step-gradient nanocomposite (NC) hydrogel generated easily by spatial connection of different nanocomposite hydrogel pastes varying in the concentrations of nanomaterials with the aid of a 3D printing technique. The prepared 3D printed gradient NC hydrogel has self-adhesive properties and is used to direct the migration of fibroblast cells towards the higher concentration of biopolymer-coated silica-based nanomaterials (NMs) within the 3D network of the hydrogel. Furthermore, we demonstrate the potential application of our gradient NC hydrogel in migration and subsequent enhanced osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBM MSC). The osteogenic differentiation of hBM MSC is achieved in the absence of osteogenic differentiation medium due to the silica-based NMs. The increase in the NM content in the gradient construct promotes hBM MSC migration and results in higher Ca2+ deposition.
In this chapter we discus molecular imprinting technology MIT , molecular imprinted polymers MIPs , and their compatibility on a proper transducer to construct a sensing system. Molecularly imprinted sensors MISens , in other words, artificial receptor-based sensors synthesized in the presence of the target molecule, are capable of sensing target molecules by using their specific cavities and are compatible with the target molecule. This MIP technology is a viable alternative of artificial receptor technology, and the sensor technology is capable of detecting any kind of molecule without pre-analytic preparations. In this chapter, you can find examples, sensor construction techniques and fundamentals of MIP and sensor combinations to look forward in your studies. For sensor technology, we explained and discussed the new sensing technologies of MIP-based electrochemical, optical especially surface plasmon resonance, SPR , and piezoelectric techniques. Therefore, this chapter presents a short guideline of MISens.
In this paper, a novel electrochemical sensor based on molecularly imprinted polypyrrole film at gold electrode for selective and sensitive detection of tyrosine (Tyr) was fabricated. Tyr was used as template molecule and polymerized in presence of pyrrole, used as the functional monomer. As a comparison a non-modified polymer surface was also prepared under same circumstances without template molecule. The feature of both imprinted and non-imprinted electrode surfaces were performed by cyclic voltammetry (CV), square wave voltammetry (SWV) and electro impedance spectroscopy (EIS). The imprinted polypyrrole sensor exhibited good selectivity toward tyrosine in comparison with other structurally similar molecules. Under the optimal experimental conditions, the linearity range and the detection limit of the imprinted sensor were obtained as 5.0x10 -9 -2.5x10 -8 M and 2.5x10 -9 M, respectively.
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