Laboratory methods are essential for the diagnosis of Mycoplasmal infection. There are three laboratory approaches are essential for the diagnosis of Mycoplasmal infection in chicken including direct methods by culture method and polymerase chain reaction, and indirect methods by detection of Mycoplasmal antibodies by serological tests. This study aimed to detection of Mycoplasma by culture and PCR technique. Two hundred seventy-six samples were collected from infected adult boiler chicken in Salah Al-din province which suffering from respiratory signs and /or joint infection, 202 respiratory and 74 articular samples. According to the results of culture, Mycoplasma isolated in rate of 35.1% (36.6% from respiratory samples and 31.1% from articular samples). The sensitivity of culture was 100%, while the specificity of culture was 97.9% when comparing with PCR results. The current study concluded that the respiratory infection was more than articular infections, and Mycoplasma gallisepticum more distributed than Mycoplasma synoviae among chickens.

The antimicrobial resistance currently impedes and threatens the future of effective prevention and treatment of the continually expanding range of infections caused by bacteria. This study aimed to identify the bacterial causes the wound infection among animals and using the antibiotic/nanoparticles mixture as a new attempt for the treatment the wound infection induced in rats. For this purpose, 112 swabs wound infection cases in the different animal types (36 sheep, 21 goats, 12 cows, 4 horses, 8 dogs, 9 rabbits, 7 genies pigs and 15 rats) were studied in the for bacterial isolation. The Pseudomonas aeruginosa was tested for its sensitivity to the antibiotics and the nanoparticles (CoFe2O4 and NiFe2O4) in vitro by using the MIC method. Also the wound infection was induced in the rats and the effect of nanoparticles/antibiotics mixture were tested in vivo. The results showed that P. aeruginosa was the predominant bacterial type that the caused wound infection. The minimum inhibitor concentration of NiFe2O4 and CoFe2O4 nanoparticles were 32 µg /ml and 16 µg /ml respectively. A clear synergistic effect of antibiotic/ nanoparticles as antibacterial were noticed which appear as a decrease in MIC and increase of the inhibitory diameter zone. According to the result of Random Amplification of Polymorphic DNA test, the nanoparticles effects on genetic material of P. aeruginosa observed as an appearance/disappearance of bands, increase in thickness and clarity of the bands.

A variety of diarrheagenic E coli (DEC) are responsible for causing diarrhea in children under five years age especially in developing courtiers. The aim of this study was to isolate and bacteriological characterizing of DEC from diarrheic children's stool and using molecular identification of DEC pathotypes for better discrimination and investigate their antibiotic resistance patterns. Total of 200 fresh stools specimens were collected from children with diarrhea in Salah Aldin Hospital in Tikrit city, Iraq. The samples were cultured on selective media such as (MacConkey and EMB). Colonies were identified through biochemical reaction and api 20E system and then antibiotic susceptibility profiles were determined. E. coli were isolated and characterized from 200 diarrheagenic stool positive samples, collected from hospitalized children less than 5 years old. A total of 75(40.5%) samples were yielded positive for growth of E. coli Out of these, 46 genotypically-identified DEC were then subjected to multiplex PCR assay targeting certain virulence factors ( SHIG, bfpA, eae, LT, ST, EA, vt1, vt2, daaE, uidA ) for discrimination of pathotyes. 21/46(45.7%) EPEC with 18 atypical and 3 typical, 11/46(24%) ETEC, 3/46 /(6.5%) EAEC, 2/46 (4.3%) for EIEC was detected, 2/46(4.3%) EHEC was also detected, while no DAEC was detected, Also mix pathotypes were detected, more than one pathotype was observed in a number of samples 7/46(15.2%), 5 (aEPEC + ETEC), 2 (aEPEC + EAEC). Moreover, all pathotypes expressed high resistant to Cefotaxime, ampicillin, Piperacillin, Azitromycin, Amikacin. while little resistance to Imipenem, Meropenem, Ciprofloxacin, Norfloxacin was observed. The study concludes EPEC is the dominant pathotype between DEC pathotypes in our society that causes diarrhea in children, and emphasizes the importance of using mPCR assay for best discrimination.

Sepsis is a leading cause of death and morbidity in newborns, especially those with very low birth weight and premature babies. The aim of our study diagnoses bacterial neonatal septicemia by direct techniques and some immunological biomarkers. The present study would include 75 neonates with clinically suspected neonatal sepsis and 50 healthy controls. Interleukin -6 levels of the blood were significantly higher in the suspected neonatal septicemia group compared with the control group. A significant increase in IL-6 levels in the blood can be used to make diagnose sepsis early.

T HIS study carried out in Salah aldeen province in period from January to September 2019. The aims of this study were to investigate incidence of Salmonella typhimurium and Salmonella dublin in local Iraqi cows by using of PCR test.The results of current study showed that the rate of isolated Salmonella species was 13.3% by culture methods, highest of them from aborted cows in rate of 22.2%.PCR test detected Salmonella typhimurium and Salmonella dublin in rate of 55% and 25% respectively, while other Salmonella species has been detected in rate of 20% from total Salmonella isolates.We can concluded from this result that highly incidence of Salmonella in cow and Salmonella typhimurium is most isolated type.