Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was analyzed by qRT PCR during abiotic stresses viz., drought, heavy metal (CdCl 2 ) and cold. Maximum expression of SAMS gene in the leaves were observed at 3 days after drought stress with 15% PEG. Conversely, its expression was not detected in leaves and roots at cadmium stress but transcripts were down regulated as compared to the control. After 6 days of stress expression of SAMS gene was increased in leaves and roots as compared to the control but it was lower than its expression at 3 days after stress. The activities of antioxidative enzymes like glutathione reductase, glutathione-s-transferase, ascorbate peroxidase and metabolite constituents like polyamines and glycine betaine were also analyzed. The activities of antioxidative enzymes and concentration of glycine betaine showed remarkable increase in response to all stresses, except ascorbate peroxidase in heavy metal stress.
Sesamum indicum L. is highly vulnerable to biotic stresses in which charcoal rot disease cuased by Macrophomina phaseolina is one of the major constraints of sesame yield. In response to pathogen infection plant produces a large amount of secondary metabolites like PR proteins, phenolics and other signaling compounds. In present investigation the role PR proteins β-1,3 glucanases and chitinases during host-pathogen interaction was studied at different time interval i.e. 0, 24, 48 and 72 hours post inoculation. Maximum induction of β, 1-3 glucanases was found at 48hpi in resistant genotypes than the susceptible. However maximum chitinase induction was noted in susceptible genotypes at 48 hpi. The expression of both genes was studied by quantitative real time PCR which was also supported the biochemical enzyme assay.
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