Abiotic stresses alter expression of S-Adenosylmethionine synthetase gene, polyamines and antioxidant activity in pigeon pea (Cajanus cajan L.)

Abstract

Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was analyzed by qRT PCR during abiotic stresses viz., drought, heavy metal (CdCl 2 ) and cold. Maximum expression of SAMS gene in the leaves were observed at 3 days after drought stress with 15% PEG. Conversely, its expression was not detected in leaves and roots at cadmium stress but transcripts were down regulated as compared to the control. After 6 days of stress expression of SAMS gene was increased in leaves and roots as compared to the control but it was lower than its expression at 3 days after stress. The activities of antioxidative enzymes like glutathione reductase, glutathione-s-transferase, ascorbate peroxidase and metabolite constituents like polyamines and glycine betaine were also analyzed. The activities of antioxidative enzymes and concentration of glycine betaine showed remarkable increase in response to all stresses, except ascorbate peroxidase in heavy metal stress.

Meta-Analysis as a Tool to Identify Candidate Genes Involved in the Fagus sylvatica L. Abiotic Stress Response

“…From the observation of our results, SAM seems to be up-regulated during cold stress (Table 2, Table S4). Studies of Guo et al [74], Mahatma et al [75], and Heidari et al [76] demonstrated that in plants such as Medicago sativa L. [74], Cajanus cajan L. [75], and Solanum lycopersicum L. [76], a gene encoding for SAMS was up-regulated in response to cold stress. Induction of SAMS during stress increases SAMS levels as positive effects of ethylene and polyamine biosynthesis [71].…”

“…Our results showed that regardless of the species subjected to abiotic stresses, the 12 genes analysed are involved in response to abiotic stress (Table S4). In fact, LHC-II was involved in plants such as tobacco and tomato in response to cold stress and SAM was involved in Medicago sativa L. [74], Cajanus cajan L. [75], and Solanum lycopersicum L. [76] (Table S3). [52]; HPT/AHP2 Histidine-containing phosphotransfer ABF [52]; OSCA1.1, Hyperosmolality-gated Ca 2+ permeable channel 1.1 [5,103]; OSCA1.2, Hyperosmolality-gated Ca 2+ permeable channel 1.2 [5,102]; OSCA3.1, Hyperosmolality-gated Ca 2+ permeable channel 3.1 [5,102]; AHA2, inhibition of the plasma membrane H + -ATPase 2 [52,104]; RALFs, Rapid alkalinization factors [5,105]; IPUT1, Inositol phosphorylceramide glucuronosyltransferase [5,106]; GIPC, Sphingolipid glycosyl insitol phosphorylceramide [5,106]; LLG1, co-receptor LORELEI-like GPI-anchored protein 1 [5,107,108]; LRX3, Leucinerich extension 3 [5,107,108]; LRX4, Leucine-rich extension 4 [5,107,108]; LRX5, Leucine-rich extension 5 [5,107,108]; SIP, RALF22 ligand via Site 1 Protease [5,109]; MYB, MYB-type transcription factor [110]; TCP, TCP-type transcription factor [110]; Class III HD-ZIP, Class III HD-ZIP transcription factor [110]; CBF-B/NF-YA, CCAAT-binding trans...…”

Polyamines and Legumes: Joint Stories of Stress, Nitrogen Fixation and Environment

“…Heavy metal stress induced the accumulation of Spd in Cajanus cajan (Radadiya et al, 2016) and of Put, Spm, and Spd in V. radiata (Choudhary and Singh, 2000). In G. max , the involvement of PAs seedlings response to cadmium stress was revealed by the induction of SAMDC after 3 and 24 h of Cd treatment (Chmielowska-Bąk et al, 2013).…”