Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from Psorospermum glaberrimum, it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy, and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine (Mad), emodin (Emo), quinizarin (Qui), as well as lapachol (Lap) and fraxetin (Fra) were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoid-derived metabolites and related complexes with cations and proteins. In addition, our study validates the ability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites. These results pave the way for new hypotheses on anthranoid metabolism in plants.

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ResumoAfetos veiculam uma intensidade de marcadores sociais e de subjetividade. Com isso, tornam-se altamente lucrativos para as indústrias que se alimentam das capacidades do corpo. Recorrendo a notas etnográficas sobre a Visibly Hot, uma empresa brasileira de óculos, este artigo foca as seguintes questões: com quais instrumentos essa marca regula desejos e aspirações para promover seus produtos e cativar seus funcionários? E, como os próprios funcionários participam e manipulam esse processo? Por um lado, o slogan de "ser diferente" promove uma pluralização de subjetividades, efetuando um controle avançado de identidades sexuais e raciais. Por outro lado, os vendedores acabam aprendendo suas próprias técnicas afetivas (identificação com a marca, incitação sexual) que, uma vez postas em movimento, simultaneamente aumentam e perturbam o fluxo normatizado pelas vendas.