Knowledge of normal organ weights gives the doctors idea about of whether the organ is normal or not. So, it is very important for autopsy surgeons to know the organ weights. But, Organ weights probably depend on part on body weight of the individual. So, current study was undertaken at Mortuary complex of S.S.G. Hospital, Baroda during 1 st July 2013 to 30 th June 2015. Total 200 cases based on inclusion and exclusion criteria were selected and subjected to statistical analysis. Results obtained were compared with earlier studies. Right lung weight and Left Lung weight was noted to be 0.89%-0.68% and 0.86%-0.65% of body weight respectively, which is lower than the textbooks. This type of variations demands another research.
Paradactylogyrus are small monogenean ectoparasites found mostly on the gills of Cyprinidae and are highly host specific. Conventionally these parasites are identified by microscopic analysis. In contrast to this conventional method, molecular identification using nuclear ribosomal DNA (rDNA) provides specific, sensitive and safe detection of parasites. The present study is aimed to identify the Paradactylogyrus on Catla catla using morphological and molecular tools. Morphologically the parasite is having hamuli, marginal hooks, bars and copulatory organs which differentiate it from other monogeneans. The parasite is having unpaired central spine, tubular cirrus and extracaecal vitellaria. 28s rDNA study revealed that the species is P. catlaius and the sequence was submitted to Genbank (accession no. KF269531). The study suggests that morphological characters along with molecular study are essential for validating and identifying the monogeneans. This is the first published sequence of species from the genus Paradactylogyrus (Thapar 1948).
A short term (20 days) cohabitation infection study of the gill fluke Paradactylogyrus catlaius infecting Catla catla was conducted to investigate on the mode, intensity and effect of infection on selected haematological, immunological and serum biochemical parameters in the infected fish. The results revealed that the increase in the number of parasites is rampant and increased to 161±23 parasites per gill lamella in just 20 days post-challenge. Further, significant decrease (p≤0.05) in haemoglobin (Hb), total erythrocyte count (TEC), lysozyme activity, bactericidal activity, total protein and albumin content was noticed whereas a significant increase in total leucocyte count (TLC) was recorded. Globulin content and superoxide anion production increased significantly initially and then decreased (p≤0.05). From this study, it was found that P. catlaius infection causes no mortality in catla till the 20 th day of challenge but the infection can spread to the whole population of catla in short time. Further, it was also observed that the infection can adversely affect the physiological and immunological status of the infected fish. Therefore, appropriate management and control strategies need to be formulated to prevent its spread and to avoid production loss.
A short term (20 days) cohabitation infection study of the gill fluke Paradactylogyrus catlaius infecting Catla catla was conducted to investigate on the mode, intensity and effect of infection on selected haematological, immunological and serum biochemical parameters in the infected fish. The results revealed that the increase in the number of parasites is rampant and increased to 161±23 parasites per gill lamella in just 20 days post-challenge. Further, significant decrease (p≤0.05) in haemoglobin (Hb), total erythrocyte count (TEC), lysozyme activity, bactericidal activity, total protein and albumin content was noticed whereas a significant increase in total leucocyte count (TLC) was recorded. Globulin content and superoxide anion production increased significantly initially and then decreased (p≤0.05). From this study, it was found that P. catlaius infection causes no mortality in catla till the 20 th day of challenge but the infection can spread to the whole population of catla in short time. Further, it was also observed that the infection can adversely affect the physiological and immunological status of the infected fish. Therefore, appropriate management and control strategies need to be formulated to prevent its spread and to avoid production loss.
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