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SUKORINI HENIK, SANGCHOTE SOMSIRI, KHEWKHOM NETNAPIS: Plant crude extracts and yeast as alternative to synthetic fungicide for controlling postharvest green mould on citrus fruit. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 3, pp. 795-801 The objectives of this research was to evaluate the eff ectiveness of the combination of plant crude extracts and yeasts as an alternative to replace the synthetic fungicide to control green mould rot caused by Penicillium digitatum on citrus fruit. This pathogen caused 90% of citrus losses during the storage. Control of this pathogen mainly with chemicals, but concerned with environmental contamination, human health, and pathogen resistance, chemical treatment is frequently decreased. Eugenia caryophylata crude extracts and Candica utilis showed to be the best combination to attain a reduction in green mould incidence by 90.3% and disease severity by 96.26%. Furthermore, the combination of E. caryophylata crude extracts and C. utilis had a more potent antifungal activity than imazalil. The eff ectiveness of the combination of plant crude extractss and yeasts can be an alternative treatment to replace the synthetic fungicide to control P.digitatum on citrus fruit, but the application in the packaging line needs further investigation. citrus fruit, green mould, plant crude extracts, yeast, Penicillium digitatum

Fruit rot disease is very damaging to cacao pods, which is caused by Phytoptora palmivora. The attack rate of P. palmivora varies. In Java, losses due to this disease reduce yields by 90 %. P. palmivora is a soil–borne pathogen. It is currently included in the Kingdom Chromista. Control with fungicides is not successful at this time, the alternatives is biologis control with Trichoderma sp. This research used a Completely Randomized Design (CRD) which was arranged in factorial with two factors. The first factor was Trichoderma sp. the second factor was P. palmivora. All treatment combinations were repeated three times. Trichoderma sp. antagonist test to P. palmivora was analyzed using Analysis of Variance (ANOVA) and then further tested using a 5 % BNJ. Trichoderma sp. origin from Jember and Trenggalek districts, East Java, Indonesia were able to act as antagonists against P. palmivora with the highest inhibitory of 78 %. In comparison, the lowest inhibitory was 70 % of isolates from Jember district, East java, Indoe. Characteristics of Trichoderma sp. The origin of Trenggalek Regency and Jember Regency, East Java, Indonesia in inhibiting the growth of P. palmivora has the same species, namely Trichoderma harzianum.

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