Acute and subacute 2,4-D toxicity to carp (Cyprinus carpio L.) were investigated. Acute toxicity (LC-50) was investigated in semi-static test during a 24, 48, and 96-hours exposition. Subacute toxicity was investigated by exposing fish to different 2,4,-D concentrations (150, 200, and 250 mg/L) for 14 days. Biochemical and morphological changes in certain organs and tissues were investigated. LC-50 values at 24 hours exposure was 310.0 mg/L, 295.0 mg/L for 48 hours, and 270.0 mg/L for 96 hours exposure.Subacute toxicity tests show that 2,4-D induce changes in the enzyme activities (AP, GOT, GPT) and morphological changes in the gills, liver and kidneys, but changes are of limited biological importance.
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