A method was developed whereby high quality FTIR spectra could be rapidly acquired from soil-borne filamentous cyanobacteria using ATR FTIR spectroscopy. Spectra of all strains displayed bands typical of those previously reported for microalgae and water-borne cyanobacteria, with each strain having a unique spectral profile. Most variation between strains occurred in the C-O stretching and the amide regions. Soft Independent Modelling by Class Analogy (SIMCA) was used to classify the strains with an accuracy of better than 93%, with best classification results using the spectral region from 1800-950 cm(-1). Despite this spectral region undergoing substantial changes, particularly in amide and C-O stretching bands, as cultures progressed through the early-, mid- to late-exponential growth phases, classification accuracy was still good (approximately 80%) with data from all growth phases combined. These results indicate that ATR/FTIR spectroscopy combined with chemometric classification methods constitute a rapid, reproducible, and potentially automated approach to classifying soil-borne filamentous cyanobacteria.
The plankton communities (phytoplankton and zooplankton) and physicochemical properties in seawater were monitored between January to December 2008 at nine coastal mariculture and local fisheries piers in Phang Nga Province, Thailand, to assess water quality rehabilitation following the Tsunami disaster of 2004. All physical and chemical values fell within the ranges that are considered normal for unpolluted water: temperature ranged from 29.84 (± 0.47) - 31.29 (± 0.27) °C; transparency ranged from 49 (± 7) - 108 (± 7) cm; total dissolved solids ranged from 17,060 (± 162) - 26,240 (± 68) mg/l; electrical conductivity ranged from 34.12 (± 3.48) - 52.48 (± 1.62) mS/cm; pH ranged from 6.69 (± 0.13) - 7.94 (± 0.07); salinity ranged from 19.48 (± 1.78) - 30.28 (± 0.44) ppt.; dissolved oxygen ranged from 6.36 (± 0.35) - 7.49 (± 0.35); biochemical oxygen demand ranged from 2.04 (± 0.25) - 3.80 (± 0.20) mg/l; and chemical oxygen demand ranged from 34.0 (± 0.95) - 63.3 (± 0.84) mg/l. Phytoplanktons were recorded to Euglenophyta, Chlorophyta, Chrysophyta, Cyanophyta and Pyrrhophyta. Zooplanktons were determined to six Phyla i.e. Chordata, Annelida, Arthropoda, Rotifera, Mollusca and Protozoa.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.