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Not all antibodies against SARS-CoV-2 inhibit viral entry, and hence, infection. Neutralizing antibodies are more likely to reflect real immunity; however, certain tests investigate protein/protein interaction rather than the fusion event. Viral and pseudoviral entry assays detect functionally active antibodies but are limited by biosafety and standardization issues. We have developed a Spike/ACE2-dependent fusion assay, based on a split luciferase. Hela cells stably transduced with Spike and a large fragment of luciferase were co-cultured with Hela cells transduced with ACE2 and the complementary small fragment of luciferase. Cell fusion occurred rapidly allowing the measurement of luminescence. Light emission was abolished in the absence of Spike and reduced in the presence of proteases. Sera from COVID-19-negative, non-vaccinated individuals or from patients at the moment of first symptoms did not lead to a significant reduction of fusion. Sera from COVID-19-positive patients as well as from vaccinated individuals reduced the fusion. This assay was more correlated to pseudotyped-based entry assay rather than serology or competitive ELISA. In conclusion, we report a new method measuring fusion-inhibitory antibodies in serum, combining the advantage of a complete Spike/ACE2 interaction active on entry with a high degree of standardization, easily allowing automation in a standard bio-safety environment.

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the first two years was 0.26 (0.14-0.46, p,0.001), while the HR of MI was 0.31 (0.17-0.58, p,0.001) and the HR of bleeding was 0.04 (0.01-0.33, p=0.002). The HR of MACE, MI and bleeding for the subsequent eight years, as well as the HR of all-cause mortality and cardiovascular mortality, were statistically insignificant. Conclusions: The study showed that switching of newer P2Y12 receptor inhibitors and clopidogrel in DAPT was associated with fewer MACE, fewer MI and fewer bleeding in first two years post-discharge, compared to nonswitching strategy in East Asian ACS patients. There were no statistically significant differences for MACE, MI and bleeding in the subsequent eight years, nor for allcause mortality and cardiovascular mortality.

A725robust in the one-way sensibility analyses and the probabilistic sensitivity analysis indicating that the absence of monitoring strategy has the highest probability of cost-effectiveness ConClusions: Long-run WBC monitoring based on current national detection guidelines is not cost-effective, even by unrealistically high agranulocytosis prevalence. New guidelines are needed to improve WBC monitoring in schizophrenic patients receiving Clozapine. PSY2 OSteOtOmia De acOrtamientO raDial en la enfermeDaD De KienbOcK (SeguimientO De 5 añOS)Vazquez Alonso F. imss hospital de traumatologia y ortopedia lomas verdes, naucalpa edo de mexico, Mexico objeCtivos: evaluar los resultados clinicos y funcionales ( grado de satisfacción ) de paciente con enfermedad de kienbock ttratados con osteotomia de acortamiento radial. MetodologíAs: estudio realizado en la umae de traumatologia y ortopedia lomas verdes del imss, en el servicio de cirugia de mano, en un periodo comprendido entre 2000 al 2003, con seguimiento de 5 años.estudio retrospectivo, observacional, transversal. se estudiaron ´18 pacientes de ambos sexos con diagnostico de enfermedad de kienbock utilizando la clasificacion de litchman, evaluados con las escala de wrist mayo score y QDash. pba estadistica de wiscolson ResultAdos: se obtuviero 18 paciente 9 mujeres y 9 hombres, el 84% obreros 16% al hogar, de acuerdo al resultados de la escla funcional wrist mayo score fueron 56% buenos, 33% excelentes, 5.5% malo, 5% regulares, con 25 puntos de grado de satifaccion de acuerdo al QDash. un paciente fue reintervenido 2 años posteriores al cual se le realizo una artrodesis delas cuatro esquinas. ConClusiones: la evaluacion clinica y funcional, sugiere una eficacia aceptable de la osteotomia de acortamiento radial y provee datos alentadorespara continuar con este tipo de tratamiento. PSY3 the relatiOnShiP between SPecific annual bleeD rateS anD healthOutcOmeS amOng chilDren with Severe hemOPhilia a in latin america